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Table 1.

Thirty most abundant cDNA clusters from an adult female Ixodes scapularis salivary gland library

Cluster numberNumber of sequencesBest BlastX match to NR database1E-value2Species of NR matchBest Rpsblast to CDD database3E-value2SignalP4
44 gi|15428308| 14 kDa salivary... 7E-34 Ixodes scapularis pfam02414 Borrelia_orfA 1E-09 SIG 
41    pfam02326 YMF19 0.0005 SIG 
34 gi|5835682|gi|5835682|ref|NP_008498.1|CO 6E-83 Ixodes hexagonus pfam00115 COX1 1E-90 NoORF 
20 gi|15428348| Salp10 [Ixodes s... 5E-34 Ixodes scapularis pfam00014 Kunitz_BPTI 0.001 SIG 
18    pfam01028 Topoisomerase_I 0.0003 SIG 
14      NoORF 
14 gi|124810|IP52_ANESU PROTEASE INHIBITOR 0.01 Anemonia sulcata Smart smart00131 KU 1E-06 SIG 
11      NoORF 
gi|15428310| 25 kDa salivary... 1E-105 Ixodes scapularis   SIG 
10 gi|5835692|gi|5835692|ref|NP_008508.1|CY 2E-73 Ixodes hexagonus pfam00033 cytochrome_b_N 1E-38 NoORF 
11 gi|5835683|gi|5835683|ref|NP_008499.1|CO 6E-37 Ixodes hexagonus pfam00116 COX2 5E-26 NoORF 
12 gi|5835693|gi|5835693|ref|NP_008509.1|ND 1E-50 Ixodes hexagonus pfam00146 NADHdh 2E-52 NoORF 
13 gi|7302028| CG1746 gene product 7E-17 Drosophila melanogaster pfam00137 ATP-synt_C 8E-20 ANCH 
14      ANCH 
15      NOSIG 
16    Smart smart00131 KU 4E-05 ANCH 
17 gi|3881447| contains similarity to Pfam 0.018  Smart smart00131 KU 0.0001 NoORF 
18    pfam02098 His_binding 0.0007 SIG 
19 gi|3451024| cytochrome oxidase III 2E-25 Ixodes pacificus pfam00510 COX3 1E-48 NoORF 
20 gi|15428348| Salp10 [Ixodes s... 9E-14 Ixodes scapularis   SIG 
21 gi|7861934| Ke3 [Danio rerio] 2E-37 Danio rerio pfam00416 Ribosomal_S13 6E-22 NOSIG 
22 gi|8072217| Dscam [Drosophila... 0.056 Drosophila melanogaster   SIG 
23      SIG 
24    Smart smart00131 KU 0.007 SIG 
25    pfam01943 Polysacc_synt 4E-08 NoORF 
26 gi|7506657|gi|7506657|pir‖T32060 0.00001 Caenorhabditis elegans Smart smart00131 KU 2E-09 NoORF 
27 gi|15428300| 20 kDa salivary... 3E-62 Ixodes scapularis   NoORF 
28    pfam01490 Aa_trans 0.01 NoORF 
29    pfam01391 Collagen 0.0007 SIG 
30    pfam00014 Kunitz_BPTI 0.009 SIG 
Cluster numberNumber of sequencesBest BlastX match to NR database1E-value2Species of NR matchBest Rpsblast to CDD database3E-value2SignalP4
44 gi|15428308| 14 kDa salivary... 7E-34 Ixodes scapularis pfam02414 Borrelia_orfA 1E-09 SIG 
41    pfam02326 YMF19 0.0005 SIG 
34 gi|5835682|gi|5835682|ref|NP_008498.1|CO 6E-83 Ixodes hexagonus pfam00115 COX1 1E-90 NoORF 
20 gi|15428348| Salp10 [Ixodes s... 5E-34 Ixodes scapularis pfam00014 Kunitz_BPTI 0.001 SIG 
18    pfam01028 Topoisomerase_I 0.0003 SIG 
14      NoORF 
14 gi|124810|IP52_ANESU PROTEASE INHIBITOR 0.01 Anemonia sulcata Smart smart00131 KU 1E-06 SIG 
11      NoORF 
gi|15428310| 25 kDa salivary... 1E-105 Ixodes scapularis   SIG 
10 gi|5835692|gi|5835692|ref|NP_008508.1|CY 2E-73 Ixodes hexagonus pfam00033 cytochrome_b_N 1E-38 NoORF 
11 gi|5835683|gi|5835683|ref|NP_008499.1|CO 6E-37 Ixodes hexagonus pfam00116 COX2 5E-26 NoORF 
12 gi|5835693|gi|5835693|ref|NP_008509.1|ND 1E-50 Ixodes hexagonus pfam00146 NADHdh 2E-52 NoORF 
13 gi|7302028| CG1746 gene product 7E-17 Drosophila melanogaster pfam00137 ATP-synt_C 8E-20 ANCH 
14      ANCH 
15      NOSIG 
16    Smart smart00131 KU 4E-05 ANCH 
17 gi|3881447| contains similarity to Pfam 0.018  Smart smart00131 KU 0.0001 NoORF 
18    pfam02098 His_binding 0.0007 SIG 
19 gi|3451024| cytochrome oxidase III 2E-25 Ixodes pacificus pfam00510 COX3 1E-48 NoORF 
20 gi|15428348| Salp10 [Ixodes s... 9E-14 Ixodes scapularis   SIG 
21 gi|7861934| Ke3 [Danio rerio] 2E-37 Danio rerio pfam00416 Ribosomal_S13 6E-22 NOSIG 
22 gi|8072217| Dscam [Drosophila... 0.056 Drosophila melanogaster   SIG 
23      SIG 
24    Smart smart00131 KU 0.007 SIG 
25    pfam01943 Polysacc_synt 4E-08 NoORF 
26 gi|7506657|gi|7506657|pir‖T32060 0.00001 Caenorhabditis elegans Smart smart00131 KU 2E-09 NoORF 
27 gi|15428300| 20 kDa salivary... 3E-62 Ixodes scapularis   NoORF 
28    pfam01490 Aa_trans 0.01 NoORF 
29    pfam01391 Collagen 0.0007 SIG 
30    pfam00014 Kunitz_BPTI 0.009 SIG 

SIG, secretory signal sequence found; NoOrf, no suitable frame or start methionine was found; NoSIG, no secretory signal is found; ANCH, membrane anchor sequence found.

1

BlastX performed with Blosum62 matrix and an E-value cutoff of 0.1 against the non-redundant (NR) protein database of NCBI.

2

Indicates significance of match to NR or CDD sequence of previous column.

3

RPSblast performed against the conserved domains database (CDD) of NCBI containing all PFAM and Smart motifs.

4

Combined results from all sequences after submission of best open reading frame to the SignalP server (Ref***)

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