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Table 3.

Effects of four ATPase inhibitors on Riftia pachyptila proton-equivalent elimination rates

Number of worms exposedProton elimination rate (μmol lg-1 h-1)Percentage reduction in proton elimination rate post-exposure
InhibitorPre-exposurePost-exposureInhibitor targets
Amiloride 16.44±0.88 (14) 0.59±0.51 (11) All ATPases 96.5 
Lansoprazole 14.3±3.9 (9) 11.9±5.2 (15) K+/H+-ATPases 17.8 
Vanadate 12.6±3.6 (19) -0.05±0.05 (8) All ATPases 100 
N-ethyl-maleimide 93.7±13.0 (17) 3.33±0.49 (13) All ATPases 96.4 
Number of worms exposedProton elimination rate (μmol lg-1 h-1)Percentage reduction in proton elimination rate post-exposure
InhibitorPre-exposurePost-exposureInhibitor targets
Amiloride 16.44±0.88 (14) 0.59±0.51 (11) All ATPases 96.5 
Lansoprazole 14.3±3.9 (9) 11.9±5.2 (15) K+/H+-ATPases 17.8 
Vanadate 12.6±3.6 (19) -0.05±0.05 (8) All ATPases 100 
N-ethyl-maleimide 93.7±13.0 (17) 3.33±0.49 (13) All ATPases 96.4 

Worms were maintained until autotrophy had been established. Inhibitors were then introduced into the seawater by injection prior to the high-pressure seawater pumps. Worms were exposed to inhibitors for at least 6h. Changes in seawater pH (i.e. the difference in the seawater pH between the control and experimental aquaria) were used to calculate proton elimination rates before and after exposure to the inhibitors.

Values are means ± 1 S.E.M. (N). N is the number of seawater samples used in the determination of the mean.

All rates are expressed in terms of wet mass.

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