The Company of Biologists

Table 3.

The effect of washing with water (control), hexane, acetone and methanol/chloroform (1:2) on rates of water loss for prepupae of six cynipid species

		Rate of water loss (mg h^-1 cm^-2)
Species	Site of gall inducement	Water	Acetone	Hexane	Methanol/chloroform
Supranivean species
Diplolepis spinosa SK	Stem	0.24±0.04^a	0.29±0.07^a	1.18±0.12^a	6.64±1.64^b
D. spinosa AB	Stem	0.14±0.02^a	0.14±0.02^a	0.81±0.07^b	0.95±0.31^c
Periclistus pirata	Stem	0.35±0.08^a	0.60±0.10^a	5.56±1.77^b	16.6±0.52^c
Subnivean species
Periclistus sp.	Leaf	0.28±0.03^a	0.26±0.06^a	0.76±0.14^a	10.6±2.24^b
D. polita	Leaf	0.20±0.03^a	0.26±0.05^a	0.65±0.09^b	0.64±0.41^b
D. gracilis	Leaf	0.14±0.01^a	0.32±0.03^a	4.48±0.42^b	8.76±0.47^c
D. radicum	Shoot	0.23±0.01^a	0.32±0.04^a	5.85±3.48^b	13.7±1.37^c

		Rate of water loss (mg h^-1 cm^-2)
Species	Site of gall inducement	Water	Acetone	Hexane	Methanol/chloroform
Supranivean species
Diplolepis spinosa SK	Stem	0.24±0.04^a	0.29±0.07^a	1.18±0.12^a	6.64±1.64^b
D. spinosa AB	Stem	0.14±0.02^a	0.14±0.02^a	0.81±0.07^b	0.95±0.31^c
Periclistus pirata	Stem	0.35±0.08^a	0.60±0.10^a	5.56±1.77^b	16.6±0.52^c
Subnivean species
Periclistus sp.	Leaf	0.28±0.03^a	0.26±0.06^a	0.76±0.14^a	10.6±2.24^b
D. polita	Leaf	0.20±0.03^a	0.26±0.05^a	0.65±0.09^b	0.64±0.41^b
D. gracilis	Leaf	0.14±0.01^a	0.32±0.03^a	4.48±0.42^b	8.76±0.47^c
D. radicum	Shoot	0.23±0.01^a	0.32±0.04^a	5.85±3.48^b	13.7±1.37^c

Values are means ± S.E.M., N=5.

Values within a row not sharing the same superscript letter were significantly different.

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