The Company of Biologists

Table 6.

Concentrations of free amino acids (FAA) and total free amino acids(TFAA) in liver of various tissues of B. sinensis after exposure to NH₄Cl for 2 days

	Muscle (μmol g^-1)			Liver (μmol g^-1)
FAA	Control	+NH₄Cl	Control	+NH₄Cl	Control	+NH₄Cl
Ala	1.604±0.146	2.865±0.310(3)^{^*}	0.327±0.104	0.190±0.017	0.117±0.012	0.111±0.019
Arg	0.726±0.065	0.572±0.032	0.046±0.004	0.045±0.007	0.016±0.006	0.013±0.002
Asg	1.072±0.114	1.005±0.152	n.d	n.d.	0.028±0.005	0.010±0.001^{^*}
Asp	1.161±0.119	0.259±0.050^{^*}	0.539±0.139	0.188±0.037^{^*}	0.041±0.007(3)	0.021±0.004
Cys	n.d.	n.d.	n.d.	n.d.	0.010±0.003	0.011±0.006
Gln	2.639±0.247	8.118±0.067^{^*}	n.d.	3.307±0.327(3)	0.079±0.023	0.280±0.045(3)^{^*}
Glu	0.667±0.069	0.306±0.045^{^*}	3.803±0.013	6.984±0.233^{^*}	0.039±0.003(3)	0.040±0.002(3)
Gly	14.36±1.85	16.61±2.54	2.503±0.474	1.669±0.231	0.497±0.086	0.401±0.033
His	6.580±0.625	6.755±0.992	0.328±0.042	0.190±0.022^{^*}	0.130±0.016(3)	0.123±0.023(3)
Ile	0.081±0.012	0.049±0.004	0.022±0.002(3)	0.021±0.005	0.074±0.012	0.020±0.010^{^*}
Leu	0.144±0.018	0.097±0.008	0.042±0.003(3)	0.048±0.006(3)	0.131±0.020	0.061±0.009(3)^{^*}
Lys	6.580±0.455	5.521±0.091	0.397±0.058	0.238±0.041	0.351±0.073	0.287±0.039
Met	n.d.	n.d.	n.d.	n.d.	0.035±0.008	0.022±0.007(3)
Phe	0.130±0.005	0.096±0.014^{^*}	0.055±0.006	0.035±0.003	0.069±0.004	0.024±0.005(3)^{^*}
Pro	0.227±0.003	0.351±0.098(3)	0.129±0.024	0.121±0.019	0.090±0.056	0.019±0.004
Ser	0.906±0.042	1.156±0.154	0.084±0.013	0.050±0.006	0.038±0.005	0.026±0.004
Tau	16.70±1.46	18.30±2.44	17.00±1.12	14.17±0.42	0.387±0.074	0.321±0.041
Thr	1.290±0.129	1.337±0.302	0.352±0.078(3)	0.485±0.082	0.451±0.078(3)	0.184±0.037(3)^{^*}
Try	0.058±0.005	0.031±0.005^{^*}	0.042±0.008	0.021±0.004	0.060±0.003	0.027±0.003(3)^{^*}
Tyr	0.064±0.004	0.053±0.005	0.055±0.006	0.058±0.005	0.036±0.002	0.019±0.005(3)^{^*}
Val	0.145±0.016	0.100±0.013	0.054±0.017	0.032±0.007	0.118±0.016	0.099±0.025
TFAA	55.12±4.00	69.69±4.29^{^*}	26.62±1.76	25.27±3.06(3)	2.786±0.310	2.267±0.241

	Muscle (μmol g^-1)			Liver (μmol g^-1)
FAA	Control	+NH₄Cl	Control	+NH₄Cl	Control	+NH₄Cl
Ala	1.604±0.146	2.865±0.310(3)^{^*}	0.327±0.104	0.190±0.017	0.117±0.012	0.111±0.019
Arg	0.726±0.065	0.572±0.032	0.046±0.004	0.045±0.007	0.016±0.006	0.013±0.002
Asg	1.072±0.114	1.005±0.152	n.d	n.d.	0.028±0.005	0.010±0.001^{^*}
Asp	1.161±0.119	0.259±0.050^{^*}	0.539±0.139	0.188±0.037^{^*}	0.041±0.007(3)	0.021±0.004
Cys	n.d.	n.d.	n.d.	n.d.	0.010±0.003	0.011±0.006
Gln	2.639±0.247	8.118±0.067^{^*}	n.d.	3.307±0.327(3)	0.079±0.023	0.280±0.045(3)^{^*}
Glu	0.667±0.069	0.306±0.045^{^*}	3.803±0.013	6.984±0.233^{^*}	0.039±0.003(3)	0.040±0.002(3)
Gly	14.36±1.85	16.61±2.54	2.503±0.474	1.669±0.231	0.497±0.086	0.401±0.033
His	6.580±0.625	6.755±0.992	0.328±0.042	0.190±0.022^{^*}	0.130±0.016(3)	0.123±0.023(3)
Ile	0.081±0.012	0.049±0.004	0.022±0.002(3)	0.021±0.005	0.074±0.012	0.020±0.010^{^*}
Leu	0.144±0.018	0.097±0.008	0.042±0.003(3)	0.048±0.006(3)	0.131±0.020	0.061±0.009(3)^{^*}
Lys	6.580±0.455	5.521±0.091	0.397±0.058	0.238±0.041	0.351±0.073	0.287±0.039
Met	n.d.	n.d.	n.d.	n.d.	0.035±0.008	0.022±0.007(3)
Phe	0.130±0.005	0.096±0.014^{^*}	0.055±0.006	0.035±0.003	0.069±0.004	0.024±0.005(3)^{^*}
Pro	0.227±0.003	0.351±0.098(3)	0.129±0.024	0.121±0.019	0.090±0.056	0.019±0.004
Ser	0.906±0.042	1.156±0.154	0.084±0.013	0.050±0.006	0.038±0.005	0.026±0.004
Tau	16.70±1.46	18.30±2.44	17.00±1.12	14.17±0.42	0.387±0.074	0.321±0.041
Thr	1.290±0.129	1.337±0.302	0.352±0.078(3)	0.485±0.082	0.451±0.078(3)	0.184±0.037(3)^{^*}
Try	0.058±0.005	0.031±0.005^{^*}	0.042±0.008	0.021±0.004	0.060±0.003	0.027±0.003(3)^{^*}
Tyr	0.064±0.004	0.053±0.005	0.055±0.006	0.058±0.005	0.036±0.002	0.019±0.005(3)^{^*}
Val	0.145±0.016	0.100±0.013	0.054±0.017	0.032±0.007	0.118±0.016	0.099±0.025
TFAA	55.12±4.00	69.69±4.29^{^*}	26.62±1.76	25.27±3.06(3)	2.786±0.310	2.267±0.241

Values are means ± S.E.M. (N=4, unless indicated otherwise in parentheses).

n.d., not detectable.

Significantly different from control values (P<0.05).

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