The Company of Biologists

Table 1.

Change in glutamine synthetase activities in various tissues of B. sinensis after exposure to NH₄Cl for 48 h

	GSase activity
	μmol min^-1 g^-1 tissue					μmol min^-1 mg^-1 protein ×10^-3
Tissue	Control	+NH₄Cl	Increase (-fold)	Control	+NH₄Cl	Increase (-fold)
Liver	0.28±0.03	1.51±0.32^{1,^*}	5.4	3.7±0.5	16.4±2.9^{1,^*}	4.4
Intestine (anterior)	1.75±0.54	4.70±0.44^{^*}	2.7	21.2±5.8	55.3±8.2^{^*}	2.6
Intestine (posterior)	1.42±0.22	3.24±0.42^{^*}	2.3	21.0±5.9	39.8±4.3^{^*}	1.9
Stomach	12.15±1.47	11.39±1.07	0.9	185.5±21.9	163.8±25.5	0.9
Muscle	0.30±0.02	0.81±0.20^{^*}	2.7	4.1±0.4	10.5±2.7^{^*}	2.6

	GSase activity
	μmol min^-1 g^-1 tissue					μmol min^-1 mg^-1 protein ×10^-3
Tissue	Control	+NH₄Cl	Increase (-fold)	Control	+NH₄Cl	Increase (-fold)
Liver	0.28±0.03	1.51±0.32^{1,^*}	5.4	3.7±0.5	16.4±2.9^{1,^*}	4.4
Intestine (anterior)	1.75±0.54	4.70±0.44^{^*}	2.7	21.2±5.8	55.3±8.2^{^*}	2.6
Intestine (posterior)	1.42±0.22	3.24±0.42^{^*}	2.3	21.0±5.9	39.8±4.3^{^*}	1.9
Stomach	12.15±1.47	11.39±1.07	0.9	185.5±21.9	163.8±25.5	0.9
Muscle	0.30±0.02	0.81±0.20^{^*}	2.7	4.1±0.4	10.5±2.7^{^*}	2.6

GSase, glutamine synthetase.

Values are means ± S.E.M. (N=4); ¹N=5.

^*

Significantly different from control values (P<0.05).