Table 2.

Antibodies

AntibodyIsotypeDilutionSourceCat. #
OLIG2 Goat IgG 1:500 Santa Cruz sc-19969 
NKX2.2 Mouse IgG 1:50 DSHB, Iowa City, IA 74.5A5 
βIII-tubulin Mouse IgG 1:5000 Sigma T 8660 
GFAP Rabbit IgG 1:5000 DAKO Z0344 
NG2 Mouse IgG 1:400 BD Pharmingen 554275 
HB9 Mouse IgG 1:50 DSHB, Iowa City, IA 81.5C10 
PDGFRα Rabbit IgG 1:1000 Gift from Dr William Stallcup  
O4 Mouse IgM 1:50 Chemicon MAB345 
MBP Rabbit IgG 1:500 Chemicon AB5622 
Human nuclei Mouse IgG 1:200 Chemicon MAB1281 
AntibodyIsotypeDilutionSourceCat. #
OLIG2 Goat IgG 1:500 Santa Cruz sc-19969 
NKX2.2 Mouse IgG 1:50 DSHB, Iowa City, IA 74.5A5 
βIII-tubulin Mouse IgG 1:5000 Sigma T 8660 
GFAP Rabbit IgG 1:5000 DAKO Z0344 
NG2 Mouse IgG 1:400 BD Pharmingen 554275 
HB9 Mouse IgG 1:50 DSHB, Iowa City, IA 81.5C10 
PDGFRα Rabbit IgG 1:1000 Gift from Dr William Stallcup  
O4 Mouse IgM 1:50 Chemicon MAB345 
MBP Rabbit IgG 1:500 Chemicon AB5622 
Human nuclei Mouse IgG 1:200 Chemicon MAB1281 
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