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Table 1.

Quantitative analysis of BrdU incorporation at 48 hours of pMiw-Wnt1 electroporation

Number of pairsArea/10000 μm2
ExperimentalControlDifference
431.6 59.9 371.7 
452.7 110.6 342.1 
1764.5 189.7 1574.8 
2191.4 388.5 1802.9 
929.3 711.5 217.8 
969.9 262.0 707.9 
Mean±s.e.m. 1123.2±291.1 376.8±96.7 836.2±279.2 
Number of pairsArea/10000 μm2
ExperimentalControlDifference
431.6 59.9 371.7 
452.7 110.6 342.1 
1764.5 189.7 1574.8 
2191.4 388.5 1802.9 
929.3 711.5 217.8 
969.9 262.0 707.9 
Mean±s.e.m. 1123.2±291.1 376.8±96.7 836.2±279.2 

BrdU-positive areas from corresponding site of the experimental and control sides on the same section were extracted by the Image Analyzer (Aqua Cosmos,Hamamatsu Photonics) and compared as a pair. Six pairs from two embryos were analyzed. BrdU incorporation was significantly greater on the experimental side than on the control side (P<0.05, Student'st-test).

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