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Table 1.

Lack of pectoral fins in injected embryos

Injection sample (ng/embryo)n% of embryos lacking pectoral fins
tbx5 MO (10.0) 250 95 
tbx5 MO (5.3) 60 82 
tbx5 MO (2.0) 90 50 
wnt2b MO (10.0) 230 75 
wnt2b MO (5.0) 60 43 
wnt2b MO (2.0) 80 30 
wnt2b MO (10.0) 60 30 
+wnt2b RNA (0.1)   
wnt2b MO (10.0) 180 57 
+tbx5 RNA (0.1)   
Injection sample (ng/embryo)n% of embryos lacking pectoral fins
tbx5 MO (10.0) 250 95 
tbx5 MO (5.3) 60 82 
tbx5 MO (2.0) 90 50 
wnt2b MO (10.0) 230 75 
wnt2b MO (5.0) 60 43 
wnt2b MO (2.0) 80 30 
wnt2b MO (10.0) 60 30 
+wnt2b RNA (0.1)   
wnt2b MO (10.0) 180 57 
+tbx5 RNA (0.1)   

MO, morpholino.

Wild-type zebrafish embryos were injected with tbx5 orwnt2b morpholinos and scored for the absence of pectoral fins at 5 dpf. In rescue experiments, morpholinos and capped RNA were co-injected into embryos and also scored for fin phenotypes.

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