Lack of pectoral fins in injected embryos
| Injection sample (ng/embryo) . | n . | % of embryos lacking pectoral fins . |
|---|---|---|
| tbx5 MO (10.0) | 250 | 95 |
| tbx5 MO (5.3) | 60 | 82 |
| tbx5 MO (2.0) | 90 | 50 |
| wnt2b MO (10.0) | 230 | 75 |
| wnt2b MO (5.0) | 60 | 43 |
| wnt2b MO (2.0) | 80 | 30 |
| wnt2b MO (10.0) | 60 | 30 |
| +wnt2b RNA (0.1) | ||
| wnt2b MO (10.0) | 180 | 57 |
| +tbx5 RNA (0.1) |
| Injection sample (ng/embryo) . | n . | % of embryos lacking pectoral fins . |
|---|---|---|
| tbx5 MO (10.0) | 250 | 95 |
| tbx5 MO (5.3) | 60 | 82 |
| tbx5 MO (2.0) | 90 | 50 |
| wnt2b MO (10.0) | 230 | 75 |
| wnt2b MO (5.0) | 60 | 43 |
| wnt2b MO (2.0) | 80 | 30 |
| wnt2b MO (10.0) | 60 | 30 |
| +wnt2b RNA (0.1) | ||
| wnt2b MO (10.0) | 180 | 57 |
| +tbx5 RNA (0.1) |
MO, morpholino.
Wild-type zebrafish embryos were injected with tbx5 orwnt2b morpholinos and scored for the absence of pectoral fins at 5 dpf. In rescue experiments, morpholinos and capped RNA were co-injected into embryos and also scored for fin phenotypes.