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Table 1.

Antibodies used

AntibodySourceClone/catalogue numberDilution
Estrogen receptor (ER) Santa Cruz Biotechnology MC-20 1:100 
Progesterone receptor (PR) Santa Cruz Biotechnology C-19 1:800 
Ki67 Dako Cytomation MIB-1 1:1000 
Cleaved caspase-3 Cell Signaling Technology 9661 1:25 
Cytokeratin 6 (CK6) Covance PRB-169P 1:500 
Patched-1 (PTCH1) Santa Cruz Biotechnology H-267 1:25 
Smoothened (SMO) Santa Cruz Biotechnology N-19 1:50 
Patched-1(PTCH1)* Santa Cruz Biotechnology G-19 1:50 
Smoothened(SMO)* Santa Cruz Biotechnology C-17 1:50 
Smooth muscle actin (SMA) Dako Cytomation 1A4 1:400 
Donkey anti-goat Molecular Probes 1:200 
Donkey anti-rabbit Molecular Probes 1:200 
AntibodySourceClone/catalogue numberDilution
Estrogen receptor (ER) Santa Cruz Biotechnology MC-20 1:100 
Progesterone receptor (PR) Santa Cruz Biotechnology C-19 1:800 
Ki67 Dako Cytomation MIB-1 1:1000 
Cleaved caspase-3 Cell Signaling Technology 9661 1:25 
Cytokeratin 6 (CK6) Covance PRB-169P 1:500 
Patched-1 (PTCH1) Santa Cruz Biotechnology H-267 1:25 
Smoothened (SMO) Santa Cruz Biotechnology N-19 1:50 
Patched-1(PTCH1)* Santa Cruz Biotechnology G-19 1:50 
Smoothened(SMO)* Santa Cruz Biotechnology C-17 1:50 
Smooth muscle actin (SMA) Dako Cytomation 1A4 1:400 
Donkey anti-goat Molecular Probes 1:200 
Donkey anti-rabbit Molecular Probes 1:200 
*

Used on a subset of samples to confirm the expression patterns observed.

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