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Table 1.

Gene expression in the ectopic vesicles

Gene48 hours30 hours18 hours
Preplacode



Six1 0/3   
Six3 0/2   
Six4 0/4   
Eya1 0/6   
Eya2 0/2   
Eya4 0/3   
Dach1 0/2   
Dach2 1/2   
Dlx3 3/3   
Dlx5 4/4   
Irx1 2/2  0/3 
Irx2
 
2/2
 

 

 
Placode
 

 

 

 
Sox10 11/11 4/4 2/4 
Sox8 3/3   
Notch1 8/8   
EphA4 6/6 4/4 3/3 
Lun. fr. 6/6   
Tbx1 3/3   
Tbx3 2/2   
Pax2 5/5   
Nkx5.1 4/4   
Bmp4 5/5   
Bmp7 2/2   
Gata2 2/2   
Gene48 hours30 hours18 hours
Preplacode



Six1 0/3   
Six3 0/2   
Six4 0/4   
Eya1 0/6   
Eya2 0/2   
Eya4 0/3   
Dach1 0/2   
Dach2 1/2   
Dlx3 3/3   
Dlx5 4/4   
Irx1 2/2  0/3 
Irx2
 
2/2
 

 

 
Placode
 

 

 

 
Sox10 11/11 4/4 2/4 
Sox8 3/3   
Notch1 8/8   
EphA4 6/6 4/4 3/3 
Lun. fr. 6/6   
Tbx1 3/3   
Tbx3 2/2   
Pax2 5/5   
Nkx5.1 4/4   
Bmp4 5/5   
Bmp7 2/2   
Gata2 2/2   

Embryos were scored as positive if some of the ectopic vesicles, or some ectopic structure in the 18 hour post-electroporation, expressed the gene. Tbx1 and Tbx3 had signal only in the post-otic ectoderm. Genes were considered pre-placode if they were expressed early and believed to be important in placode formation. Genes were considered placode genes if they were expressed as the otic placode forms or later.

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