Genotypes of progeny from Tead heterozygous matings
| . | Genotype . | . | . | . | |||
|---|---|---|---|---|---|---|---|
| Progeny . | +/+ . | +/- . | -/- . | Total . | |||
| ♂ Tead4+/- × ♀ Tead4+/- | |||||||
| Viable adults | 120 | 247 | 0 | 367 | |||
| Implanted embryos (E6.5-15.5) | 24 | 47 | 0 | 71 | |||
| E3.5 embryos | 20 | 42 | (16)* | 78 | |||
| TS cell lines from E3.5 blastocysts | 14 | 22 | 0 | 36 | |||
| TS cell lines from E2.5 embryos | 7 | 5 | 0 | 12 | |||
| ES cell lines from 19 E2.5 embryos | 5 | 2 | 1 | 8 | |||
| ♂ Tead2+/- × ♀ Tead2+/- | |||||||
| Viable adults | 48 | 99 | 35 | 182 | |||
| E3.5 blastocysts | 6 | 16 | 5 | 27 | |||
| . | Genotype . | . | . | . | |||
|---|---|---|---|---|---|---|---|
| Progeny . | +/+ . | +/- . | -/- . | Total . | |||
| ♂ Tead4+/- × ♀ Tead4+/- | |||||||
| Viable adults | 120 | 247 | 0 | 367 | |||
| Implanted embryos (E6.5-15.5) | 24 | 47 | 0 | 71 | |||
| E3.5 embryos | 20 | 42 | (16)* | 78 | |||
| TS cell lines from E3.5 blastocysts | 14 | 22 | 0 | 36 | |||
| TS cell lines from E2.5 embryos | 7 | 5 | 0 | 12 | |||
| ES cell lines from 19 E2.5 embryos | 5 | 2 | 1 | 8 | |||
| ♂ Tead2+/- × ♀ Tead2+/- | |||||||
| Viable adults | 48 | 99 | 35 | 182 | |||
| E3.5 blastocysts | 6 | 16 | 5 | 27 | |||
Either Tead4+/- mice or Tead2+/-mice were mated. Embryos were recovered either at E2.5 or E3.5 days post-fertilization and tested for their ability to yield either TS or ES cells. Genotyping was performed either on the entire embryo, or on the embryoderived cell line.
All 16 of the Tead4-/- embryos at E3.5 were abnormal morulae.