PCR primers
| Marker . | Forward primer (5′-3′) . | Reverse primer (5′-3′) . |
|---|---|---|
| Tead4.1 | ATTACCTCCAACGAGTGGAGCTCT | |
| Tead4.2 | TCATTCTTTCACAAGTCGGT | |
| Tead4.20 | GATTAAAGGCTCACTCAGAGG | |
| Tead4.27 | CTCAACATACAGTTTGAAGCAC | |
| Tead4.28 | AGCTCCACTCGTTGGAGGTAAT | |
| P1 | CTAGCATTAAGGAATGTCCCGA | |
| P2 | CTCAACATACAGTTTGAAGCAC | |
| P3 | CGTATAGCATACATTATACGAAG | |
| P4 | GTGTTCTTAGAGGTACAGTCA | |
| RT-PCR primers | ||
| Tead4 | GCACCATTACCTCCAACGAG | GATCAGCTCATTCCGACCAT |
| Fgf4 | TCTACTGCAACGTGGGCATC | CTTCATGGTAGGCGACACTC |
| Nanog | AGGGTCTGCTACTGAGATGCTCTG | CAACCACTGGTTTTTCTGCCACCG |
| Sox2 | GGCAGCTACAGCATGATGCAGGAGC | CTGGTCATGGAGTTGTACTGCAGG |
| Rex1 | GACTAAGAGCTGGGACAC | TTCTGGCCACTTGTCTTTGC |
| T | CCGGTGCTGAAGGTAAATGT | TGACCGGTGGTTCCTTAGAG |
| Hnf4a | AAATGTGCAGGTGTTGACCA | AGGAGCAGCACGTCCTTAAA |
| Gapdh | CCAAGTAGTAGTACATC | TTCTTACTCCTTGGAGGC |
| Marker . | Forward primer (5′-3′) . | Reverse primer (5′-3′) . |
|---|---|---|
| Tead4.1 | ATTACCTCCAACGAGTGGAGCTCT | |
| Tead4.2 | TCATTCTTTCACAAGTCGGT | |
| Tead4.20 | GATTAAAGGCTCACTCAGAGG | |
| Tead4.27 | CTCAACATACAGTTTGAAGCAC | |
| Tead4.28 | AGCTCCACTCGTTGGAGGTAAT | |
| P1 | CTAGCATTAAGGAATGTCCCGA | |
| P2 | CTCAACATACAGTTTGAAGCAC | |
| P3 | CGTATAGCATACATTATACGAAG | |
| P4 | GTGTTCTTAGAGGTACAGTCA | |
| RT-PCR primers | ||
| Tead4 | GCACCATTACCTCCAACGAG | GATCAGCTCATTCCGACCAT |
| Fgf4 | TCTACTGCAACGTGGGCATC | CTTCATGGTAGGCGACACTC |
| Nanog | AGGGTCTGCTACTGAGATGCTCTG | CAACCACTGGTTTTTCTGCCACCG |
| Sox2 | GGCAGCTACAGCATGATGCAGGAGC | CTGGTCATGGAGTTGTACTGCAGG |
| Rex1 | GACTAAGAGCTGGGACAC | TTCTGGCCACTTGTCTTTGC |
| T | CCGGTGCTGAAGGTAAATGT | TGACCGGTGGTTCCTTAGAG |
| Hnf4a | AAATGTGCAGGTGTTGACCA | AGGAGCAGCACGTCCTTAAA |
| Gapdh | CCAAGTAGTAGTACATC | TTCTTACTCCTTGGAGGC |
Specific primers for β-actin, Oct4(Nichols et al., 1998) and Cdx2, Fgfr2 and Eomes(Strumpf et al., 2005) have been published.