Table 1.

Forward (F) and reverse (R) primers used to synthesize IP3R1 GST-fusion proteins

Primer name5′ restriction siteSequence 5′-3′
Domain 1F BamHI ACTAAAGGATCCATGTCTGACAAAATGTCG 
Domain 1R EcoRI CAAGGAATTCCCTAGTCTCAACCTACTCCGAGA 
Domain 2F BamHI ACTAAAGGATCCAACGCGCAAGAAAAAATG 
Domain 2R EcoRI CAAGGAATTCCCTAGCCTCATCACGTTACTGCC 
Domain 3F EcoRI GACGGAATTCCCTCTATCCATGGCGTTGGG 
Domain 3R XhoI CAAGCTCGAGCGTAGTCGGGCTGATAACCGCCA 
Domain 4F BamHI ACTAAAGGATCCAACGCCGCTCGCAGAGAC 
Domain 4R EcoRI CAAGGAATTCCCTAGCCTCCGGAAGGTGGTAAA 
Domain 5F XhoI GACACTCGAGCGGAGGCCGACCCTGATGAC 
Domain 5R NotI AACGCGGCCGCATAGGTCGATGATGACCCCGAA 
Domain 6F BamHI ACTAAAGGATCCACCTTTGCTGACCTGAGG 
Domain 6R EcoRI CAAGGAATTCCCTAGGGCCCGGCTGCTGTGGGTT 
Primer name5′ restriction siteSequence 5′-3′
Domain 1F BamHI ACTAAAGGATCCATGTCTGACAAAATGTCG 
Domain 1R EcoRI CAAGGAATTCCCTAGTCTCAACCTACTCCGAGA 
Domain 2F BamHI ACTAAAGGATCCAACGCGCAAGAAAAAATG 
Domain 2R EcoRI CAAGGAATTCCCTAGCCTCATCACGTTACTGCC 
Domain 3F EcoRI GACGGAATTCCCTCTATCCATGGCGTTGGG 
Domain 3R XhoI CAAGCTCGAGCGTAGTCGGGCTGATAACCGCCA 
Domain 4F BamHI ACTAAAGGATCCAACGCCGCTCGCAGAGAC 
Domain 4R EcoRI CAAGGAATTCCCTAGCCTCCGGAAGGTGGTAAA 
Domain 5F XhoI GACACTCGAGCGGAGGCCGACCCTGATGAC 
Domain 5R NotI AACGCGGCCGCATAGGTCGATGATGACCCCGAA 
Domain 6F BamHI ACTAAAGGATCCACCTTTGCTGACCTGAGG 
Domain 6R EcoRI CAAGGAATTCCCTAGGGCCCGGCTGCTGTGGGTT 

Restriction sites are underlined and the stop codons are indicated in bold.

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