Skip to Main Content
Table 1.

Quantitative effects of Foxa knockdown on endomesodermal genes

Expression*BlastulaMesenchyme blastulaEarly gastrulaLate gastrula
foxa EM, E +3.1; +1.4; NA; NA +2.0; + 1.6; +2.5; +2.1 +1.3;+1.4; +1.8; NA +2.5; +1.9; NA; NA 
gatae EM, E +2.9; +1.8; NA; NA NS; NS; NS; NS NS; NS; NS; −1.6 −1.6; −1.6; −2.5; −1.7 
endo 16 EM, E NS; +1.6; NA; NA NS; NS; NS; NS NS; NS; NS; NS −2.7; −3.2; −3.3; −2.7 
gcm SMC NS; NS; NA NS; NS; NS +2.2; +1.6; +3.0 NS; NS; NS 
hh E NE −5.8; −4.9; −3.4 NA; −2.5; −2.3 −2.7; −3.3; NA 
Expression*BlastulaMesenchyme blastulaEarly gastrulaLate gastrula
foxa EM, E +3.1; +1.4; NA; NA +2.0; + 1.6; +2.5; +2.1 +1.3;+1.4; +1.8; NA +2.5; +1.9; NA; NA 
gatae EM, E +2.9; +1.8; NA; NA NS; NS; NS; NS NS; NS; NS; −1.6 −1.6; −1.6; −2.5; −1.7 
endo 16 EM, E NS; +1.6; NA; NA NS; NS; NS; NS NS; NS; NS; NS −2.7; −3.2; −3.3; −2.7 
gcm SMC NS; NS; NA NS; NS; NS +2.2; +1.6; +3.0 NS; NS; NS 
hh E NE −5.8; −4.9; −3.4 NA; −2.5; −2.3 −2.7; −3.3; NA 

Numbers shown are ΔCT, or normalized CTdifferences between foxa MASO-injected embryos and control embryos.ΔCT is calculated as previously described(Oliveri and Davidson, 2004a). A positive number means the number of transcripts of the target gene is increased by the foxa MASO; a negative number means the number of target gene transcripts is decreased. Listed data are considered significant when ΔCT is <−1.6 or >+1.6. Smaller effects are shown as not significant (NS). NE, the gene is not normally expressed at this time point of development and the QPCR result is irrelevant; NA, result not available. Measurements carried out in independent batches of cDNA are separated by semicolon. Each result has been repeated at least in two separate injection experiments. This table shows quantitative results for genes affected by foxa knock-down during the developmental time period analyzed. Many other genes are not affected by foxa MASO at any timepoints: otx a, otx β1/2, krl, gatac, hmx, not, nrl, pks, tbr, brn 1/2/4, delta, eve, foxc, hox 11/13b, krox, wnt8, lox, bra, wnt5, delta, apoL,hnf6, lim1, dri, soxB, foxb, alx1, ets1.

*

Domain of expression in untreated embryos. EM, endomesodoerm; E, endoderm;SMC, secondary mesenchyme cells. For a detailed description of the expression pattern of the genes in the table, see text.

S. purpuratus embryos were collected at 18 hours (blastula), 23-24 hours (mesenchyme blastula), 30-34 hours (early gastrula) and 48 hours (late gastrula) postfertilization.

This number, even if below significance, shows the same trend of response to perturbation.

Close Modal

or Create an Account

Close Modal
Close Modal