Generation of Sall4-null ES cells
| . | Vectors . | . | Selection at second round . | Number of colonies . | Integration event . | . | . | |||
|---|---|---|---|---|---|---|---|---|---|---|
| Experiment . | First round . | Second round . | . | . | Random . | Retargeting . | Second allele . | |||
| 1 | pGK Neo | pGK Hyg | Hyg | 134 | 116 | 16 | 2 | |||
| 2 | pGK Hyg | pGK Neo | Neo | 115 | 68 | 44 | 3* | |||
| 3 | IRES Hyg | IRES β-geo | Neo | 20 | 12 | 8 | 0 | |||
| IRES Hyg | IRES β-geo | Neo+Hyg | 13 | 12 | 0 | 1* | ||||
| . | Vectors . | . | Selection at second round . | Number of colonies . | Integration event . | . | . | |||
|---|---|---|---|---|---|---|---|---|---|---|
| Experiment . | First round . | Second round . | . | . | Random . | Retargeting . | Second allele . | |||
| 1 | pGK Neo | pGK Hyg | Hyg | 134 | 116 | 16 | 2 | |||
| 2 | pGK Hyg | pGK Neo | Neo | 115 | 68 | 44 | 3* | |||
| 3 | IRES Hyg | IRES β-geo | Neo | 20 | 12 | 8 | 0 | |||
| IRES Hyg | IRES β-geo | Neo+Hyg | 13 | 12 | 0 | 1* | ||||
Two rounds of targeting were carried out to disrupt both the alleles of Sall4 in ES cells.
Two out of three clones in Experiment 2 and one clone in Experiment 3 were finally dominated by contaminating heterozygous cells.