SMA-9 is sufficient to act after the early stages of M lineage development for proper cell fate specification
| . | hsp-16p::sma-9C1::unc-54 3′UTR . | . | hsp-16p::sma-9C2::unc-54 3′UTR . | . | ||
|---|---|---|---|---|---|---|
| Stage at heatshock* . | n† . | Rescue‡ . | n† . | Rescue‡ . | ||
| 1-M | 52 | 25% | 56 | 2% | ||
| 2-M | 27 | 93% | 5 | 80% | ||
| 4-M | 6 | 100% | 8 | 88% | ||
| 6-M | 7 | 43% | 6 | 17% | ||
| 8-M | 3 | 0% | 3 | 0% | ||
| ≥10-M | 14 | 0% | 13 | 0% | ||
| SM | 6 | 0% | 7 | 0% | ||
| . | hsp-16p::sma-9C1::unc-54 3′UTR . | . | hsp-16p::sma-9C2::unc-54 3′UTR . | . | ||
|---|---|---|---|---|---|---|
| Stage at heatshock* . | n† . | Rescue‡ . | n† . | Rescue‡ . | ||
| 1-M | 52 | 25% | 56 | 2% | ||
| 2-M | 27 | 93% | 5 | 80% | ||
| 4-M | 6 | 100% | 8 | 88% | ||
| 6-M | 7 | 43% | 6 | 17% | ||
| 8-M | 3 | 0% | 3 | 0% | ||
| ≥10-M | 14 | 0% | 13 | 0% | ||
| SM | 6 | 0% | 7 | 0% | ||
Stage of M lineage development according to Fig. 1E.
Number of transgenic animals scored. For each construct, data was pooled from two independent transgenic lines.
Rescue was scored by counting the number of CCs present using CC::GFP in the sma-9 (cc604) background. No rescue was observed when non-transgenic animals were heat-shocked at the various stages.