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Table 2.

Transplanted wild-type cells in anterior somites rescue pectoral fin induction in nls

Pectoral fin present
Somite(n)*
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
Yes − ++ ++ ++ ++ − − − − − − − − − 
Yes − − − ++ ++ ++ − − − − − 
No ++ ++ ++ ++ ++ ++ − − − − − − 
No − − − − − − − − − − 
No − − − − − − − − − 
No − − − − − − − − − − − − − − − 
No − − − − − − − − ++ 
No − − − − − − − − − − − 
No − − − − − − − − ++ ++ ++ ++ − − − − 
No − − − − − − − − − − − − − − 
No − − − − − − − − − − 
No − − − − − − − − − − − ++ ++ ++ − ++ 
No − − − − − − − − − − − ++ ++ ++ 
No
 

 

 

 

 

 

 

 

 

 

 

 
++
 
+
 
++
 
+
 
+
 
Pectoral fin present
Somite(n)*
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
Yes − ++ ++ ++ ++ − − − − − − − − − 
Yes − − − ++ ++ ++ − − − − − 
No ++ ++ ++ ++ ++ ++ − − − − − − 
No − − − − − − − − − − 
No − − − − − − − − − 
No − − − − − − − − − − − − − − − 
No − − − − − − − − ++ 
No − − − − − − − − − − − 
No − − − − − − − − ++ ++ ++ ++ − − − − 
No − − − − − − − − − − − − − − 
No − − − − − − − − − − 
No − − − − − − − − − − − ++ ++ ++ − ++ 
No − − − − − − − − − − − ++ ++ ++ 
No
 

 

 

 

 

 

 

 

 

 

 

 
++
 
+
 
++
 
+
 
+
 

Rescue of pectoral fin development in nls embryos. nls hosts were identified by their hindbrain phenotype (otic placode abuts the first somite)at 22-24 hpf; nls hosts were scored for the number and distribution of transplanted cells, and presence of a pectoral fin bud, at 48-50 hpf.

*

Shown are transplants located in the first 16 somites; donor-derived muscle fibres were counted in somites (vertical columns), indicated as absence(−), or fewer (+) or more (++) than ten fibers per somite.

In this nls fish, all transplanted cells were located dorsal to the horizontal myoseptum.

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