The Company of Biologists

Table 4.

Comparison of drugs and mutant strains

A Drugs
Drug	Concentration (μM)	SDF-2 production upon SDF-2 priming	SDF-2 production upon GABA priming	SDF-2 production in the presence of glutamate
None		Yes	Yes	No
CGP 55845	0.001	No	No	NA
CGP54626	0.001	No	No	NA
LY294002	1	Yes	No	No
Akt inhibitor IV	1	Yes	No	No
Akt inhibitor VIII	10	Yes	Yes	No
H-89	10	No	Yes	No
B Strains
Strain	SDF-2 in sorus	SDF-2 production upon SDF-2 priming	SDF-2 production upon GABA priming	SDF-2 production in presence of glutamate
AX4	Yes	Yes	Yes	No
gadA^-	No	Yes	Yes	No
grlE^-	No	Yes	No	Yes^*
acbA^-	No	No	No	NA
pik1^-2^-	No	Yes	No	No
pkbR1^-	No	Yes	No	No
acaA^-K	Yes	Yes	Yes	No
gα 9^-	Yes	Yes	Yes	Yes

`Yes' indicates at least 5000 units of SDF-2 per 10³ cells; `No'indicates fewer than 10 units of SDF-2 per 10³ cells. The minimum effective concentration of the compounds on KP cells is given. Cells of the various strains were dissociated from culminants and treated with 1 pM SDF-2 or 10 nM GABA. pik1^-2^- and acaA^- K cells can develop if plated at high density such that they can form aggregates by accretion. To determine whether glutamate inhibited SDF-2 production in cells treated with the various drugs or in the mutant strains, 1 μM glutamate was added simultaneously with SDF-2 or GABA,and the production of SDF-2 was measured in the supernatant after 30 minutes. All experiments were repeated three times. NA, not applicable.

With SDF-2 priming but not with GABA priming.

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