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Table 4.

Comparison of drugs and mutant strains

A Drugs
 

 

 

 

 
Drug
 
Concentration (μM)
 
SDF-2 production upon SDF-2 priming
 
SDF-2 production upon GABA priming
 
SDF-2 production in the presence of glutamate
 
None  Yes Yes No 
CGP 55845 0.001 No No NA 
CGP54626 0.001 No No NA 
LY294002 Yes No No 
Akt inhibitor IV Yes No No 
Akt inhibitor VIII 10 Yes Yes No 
H-89 10 No Yes No 
B Strains
 

 

 

 

 
Strain
 
SDF-2 in sorus
 
SDF-2 production upon SDF-2 priming
 
SDF-2 production upon GABA priming
 
SDF-2 production in presence of glutamate
 
AX4 Yes Yes Yes No 
gadA- No Yes Yes No 
grlE- No Yes No Yes* 
acbA- No No No NA 
pik1-2- No Yes No No 
pkbR1- No Yes No No 
acaA-K Yes Yes Yes No 
gα 9- Yes Yes Yes Yes 
A Drugs
 

 

 

 

 
Drug
 
Concentration (μM)
 
SDF-2 production upon SDF-2 priming
 
SDF-2 production upon GABA priming
 
SDF-2 production in the presence of glutamate
 
None  Yes Yes No 
CGP 55845 0.001 No No NA 
CGP54626 0.001 No No NA 
LY294002 Yes No No 
Akt inhibitor IV Yes No No 
Akt inhibitor VIII 10 Yes Yes No 
H-89 10 No Yes No 
B Strains
 

 

 

 

 
Strain
 
SDF-2 in sorus
 
SDF-2 production upon SDF-2 priming
 
SDF-2 production upon GABA priming
 
SDF-2 production in presence of glutamate
 
AX4 Yes Yes Yes No 
gadA- No Yes Yes No 
grlE- No Yes No Yes* 
acbA- No No No NA 
pik1-2- No Yes No No 
pkbR1- No Yes No No 
acaA-K Yes Yes Yes No 
gα 9- Yes Yes Yes Yes 

`Yes' indicates at least 5000 units of SDF-2 per 103 cells; `No'indicates fewer than 10 units of SDF-2 per 103 cells. The minimum effective concentration of the compounds on KP cells is given. Cells of the various strains were dissociated from culminants and treated with 1 pM SDF-2 or 10 nM GABA. pik1-2- and acaA- K cells can develop if plated at high density such that they can form aggregates by accretion. To determine whether glutamate inhibited SDF-2 production in cells treated with the various drugs or in the mutant strains, 1 μM glutamate was added simultaneously with SDF-2 or GABA,and the production of SDF-2 was measured in the supernatant after 30 minutes. All experiments were repeated three times. NA, not applicable.

*

With SDF-2 priming but not with GABA priming.

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