Skip to Main Content
Table 2.

Explosure of the protease domain of TagC

Treatment before addition of antibodiesTreatment after removal of antibodiesSDF-2 production from recombinant AcbA (U/103 cells)
Unprimed None <10 
Unprimed Primed 12,000±1000 
Primed None 70±30 
Primed Primed 30±20 
Treatment before addition of antibodiesTreatment after removal of antibodiesSDF-2 production from recombinant AcbA (U/103 cells)
Unprimed None <10 
Unprimed Primed 12,000±1000 
Primed None 70±30 
Primed Primed 30±20 

Unprimed cells were treated with antibodies to the protease domain of TagC for 5 minutes and then extensively washed before being primed with either 10 nM GABA or 10 pM SDF-2. Primed cells were given either 10 nM GABA or 10 pM SDF-2 for 5 minutes, and then washed before adding antibodies to the protease domain of TagC. After 5 minutes, free antibody was washed away and half of the cells were primed with either GABA or SDF-2. Five minutes after priming, the cells were washed and incubated with 10 pmoles recombinant AcbA for 30 minutes before the level of SDF-2 was determined in the supernatant. All experiments were repeated at least three times.

Close Modal

or Create an Account

Close Modal
Close Modal