Inhibition of SDF-2 production upon addition of anti-TagC antibodies or ABC inhibitors
Additions . | SDF-2 production . |
---|---|
None | No |
0.1 pM SDF-2 | Yes |
0.1 pM SDF-2 + anti TagC | No |
10 nM GABA + anti TagC | No |
0.1 pM SDF-2 + 2 mM vanadate | No |
10 nM GABA + 2 mM vanadate | No |
0.1 pM SDF-2 + 100 μM verapamil | No |
10 nM GABA + 100 μM verapamil | No |
0.1 pM SDF-2 + 200 μM corticosterone | No |
10 nM GABA + 200 μM corticosterone | No |
Additions . | SDF-2 production . |
---|---|
None | No |
0.1 pM SDF-2 | Yes |
0.1 pM SDF-2 + anti TagC | No |
10 nM GABA + anti TagC | No |
0.1 pM SDF-2 + 2 mM vanadate | No |
10 nM GABA + 2 mM vanadate | No |
0.1 pM SDF-2 + 100 μM verapamil | No |
10 nM GABA + 100 μM verapamil | No |
0.1 pM SDF-2 + 200 μM corticosterone | No |
10 nM GABA + 200 μM corticosterone | No |
Developed KP cells were treated with the various compounds and the supernatants collected after 1 hour, serially diluted and added to test KP cells.
`Yes' indicates at least 5000 units of SDF-2 per 103 cells; `No'indicates fewer than 10 units of SDF-2 per 103 cells.
All experiments were repeated at least three times.