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Table 1.

Dorsalization phenotype of β4GalT5 morphant embryos and mRNA rescue

Wild typeClass 3* - mild dorsalization (similar to pgy)Class 4* - moderate dorsalization (similar to snh)Class 5* - severe dorsalization (similar to swr)Percent displaying phenotype
5 ng MO1(n=5714% (872% (41) 14% (8) 86% (49) 
10 ng MO1 (n=373) 15% (55) 13% (50) 60% (223) 12% (45) 85% (318) 
20 ng MO1 (n=78) 15% (12) 5% (4) 14% (11) 65% (51) 85% (66) 
10 ng MO2 (n=324) 19% (61) 13% (42) 38% (122) 31% (99) 81% (263) 
10 ng MO3 (n=111) 19% (21) 19% (21) 28% (31) 34% (38) 82% (90) 
10 ng MO3 + 40 pg mdrβ4GalT5 mRNA (n=118) 71% (84) 3% (3) 14% (17) 14% (16) 31% (36) 
Wild typeClass 3* - mild dorsalization (similar to pgy)Class 4* - moderate dorsalization (similar to snh)Class 5* - severe dorsalization (similar to swr)Percent displaying phenotype
5 ng MO1(n=5714% (872% (41) 14% (8) 86% (49) 
10 ng MO1 (n=373) 15% (55) 13% (50) 60% (223) 12% (45) 85% (318) 
20 ng MO1 (n=78) 15% (12) 5% (4) 14% (11) 65% (51) 85% (66) 
10 ng MO2 (n=324) 19% (61) 13% (42) 38% (122) 31% (99) 81% (263) 
10 ng MO3 (n=111) 19% (21) 19% (21) 28% (31) 34% (38) 82% (90) 
10 ng MO3 + 40 pg mdrβ4GalT5 mRNA (n=118) 71% (84) 3% (3) 14% (17) 14% (16) 31% (36) 

MO1, translation blocker (-50 bp); MO2, splice blocker (exon 1 splice donor); MO3, translation blocker (start site); mdrβ4GalT5 mRNA,MO3-binding site abolished.

*

Classified according to Mullins et al.(Mullins et al., 1996).

Total embryos treated.

Total embryos in each class.

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