The Company of Biologists

Table 1.

The majority of CCFSE-labeled mesothelial cells and their descendants co-label for β-Gal

A
	CCFSE surface				Submesothelial
0 days in culture	Total	β-Gal⁺	β-Gal^–	Total
Number of cells	102	98	4	0
Average percentage		96.1%	3.9%

A
	CCFSE surface				Submesothelial
0 days in culture	Total	β-Gal⁺	β-Gal^–	Total
Number of cells	102	98	4	0
Average percentage		96.1%	3.9%

B
		CCFSE surface					CCFSE submesothelial
1-2 days in culture	CCFSE (total)	Total	β-Gal⁺	β-Gal^–	Total	β-Gal⁺	β-Gal^–
Number of cells	488	355	301	54	133	100	33
Average percentage			88.6%	11.4%		74.4%	25.6%
Average percentage		74.5%			25.5%

B
		CCFSE surface					CCFSE submesothelial
1-2 days in culture	CCFSE (total)	Total	β-Gal⁺	β-Gal^–	Total	β-Gal⁺	β-Gal^–
Number of cells	488	355	301	54	133	100	33
Average percentage			88.6%	11.4%		74.4%	25.6%
Average percentage		74.5%			25.5%

At 0 days of culture (A), no CCFSE-labeled cells were detected in the submesothelial space.

After 1-2 days of culture (B), of a total of 488 CCFSE-marked cells counted, 133 were found in the submesothelial space (25.5%), and 355 were found in the surface mesothelium (74.5%). Of the 133 CCFSE-marked cells in the submesothelium, 100 cells were found to co-label with β-Gal(β-Gal⁺, 74.4%) and 33 cells were without β-Gal co-label(β-Gal^–, 25.6%), whereas of the 355 CCFSE-positive cells in the serosal mesothelium, 301 cells were found to co-label with β-Gal(88.6%). Cell numbers were collected from five sections (0 days in culture),and 14 sections (1-2 days in culture), and average percentages calculated.

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