A comparison of the number of muscle arms in various mutant backgrounds from body wall muscles of the dorsal right quadrant
| . | Genotype* . | Description . | Mean† . | P‡ . | P§ . |
|---|---|---|---|---|---|
| 1 | trIs30 (15°C) | Wild-type young adults | 3.8±1.1 | ns | - |
| 2 | trIs30 (20°C) | Wild-type young adults | 3.5±0.9 | - | - |
| 3 | trIs30 (25°C) | Wild-type young adults | 3.7±1.0 | ns | - |
| 4 | trIs30(ORNAi) | Wild-type young adults | 3.5±0.8 | ns | - |
| 5 | trIs30 (20°C) | Wild-type L1 hatchlings | 1.7±0.8¶ | <0.001 | - |
| 6 | sma-6(e1482) | Tgf-β receptor (small worms) | 3.4±0.8 | ns | - |
| 7 | lon-2(e678) | Glypican (long worms) | 3.7±1.2 | ns | - |
| 8 | dpy-5(e61)¶ | Collagen (short worms) | 3.6±0.7¶ | ns | - |
| 9 | dyp-10(e128) | Collagen (control for unc-104 null) | 3.6±0.9¶ | ns | - |
| 10 | act-1,2,3(st15) | Actin | 2.4±1.0¶ | <0.001 | <0.001 (5) |
| 11 | unc-60(su158) | ADF/cofilin (null) | 1.8±0.9 | <0.001 | ns (5) |
| 12 | unc-60(e723) | ADF/cofilin (hypomorph) | 1.8±0.8 | <0.001 | ns (5) |
| 13 | unc-60(s1307) | ADF/cofilin (hypermorph) | 3.3±1.0 | ns | <0.001 (11) |
| 14 | unc-60(r398) | ADF/cofilin (no severing) | 3.3±1.2 | ns | <0.001 (11) |
| 15 | unc-78(gk27) | Actin-interacting protein 1 (null) | 3.4±1.0 | ns | - |
| 16 | lev-11(TM1-RNAi) | Tropomyosin | 2.1±1.0 | <0.001 | ns (5) |
| 17 | lev-11(TM2-RNAi) | Tropomyosin | 2.2±1.1 | <0.001 | 0.038 (5) |
| 18 | act-1,2,3(st15); lev-11(TM1-RNAi) | - | 1.7±0.8¶ | <0.001 | ns (15)/ns (5) |
| 19 | unc-60(su158); lev-11(TM1-RNAi) | - | 1.4±0.6¶ | <0.001 | ns (10)/ns (5) |
| 20 | unc-54(e190) | Muscle myosin (MHC B) (null) | 2.4±1.1 | <0.001 | <0.001 (5) |
| 21 | unc-54(e190) | unc-54 L1 hatchlings (null) | 1.7±0.7¶ | <0.001 | ns (5) |
| 22 | unc-54(e1152) | Muscle myosin (MHC B) | 2.3±1.1 | <0.001 | - |
| 23 | unc-54(RNAi) | Muscle myosin (MHC B) | 2.3±1.2 | <0.001 | ns (20) |
| 24 | unc-15(e73)¶ | Paramyosin | 2.7±1.1 | <0.001 | - |
| 25 | unc-15(su2000)¶ | Paramyosin | 3.0±1.0 | <0.001 | - |
| 26 | unc-15(RNAi) | Paramyosin | 3.1±1.2 | <0.001 | 0.007 (24) |
| 27 | unc-15(e1402ts) (25°C)¶ | Paramyosin | 3.1±1.0 | 0.003 | - |
| 28 | unc-54(e190); lev-11(TM1-RNAi) | - | 2.1±0.9 | <0.001 | - |
| 29 | act-1,2,3(st15); unc-54(RNAi) | - | 2.9±0.9¶ | <0.001 | 0.037 (10) |
| 30 | unc-60(su158); unc-54(RNAi) | - | 1.6±0.8 | <0.001 | ns (11) |
| 31 | unc-104(e1265) | Kinesin | 1.7±1.2 | <0.001 | ns (5) |
| 32 | unc-104(rh43) | Kinesin | 1.3±1.0 | <0.001 | 0.011 (5) |
| 33 | unc-104(rh142) dyp-10(e128) | unc-104 null | 1.2±0.6¶ | <0.001 | <0.001 (5) |
| 34 | egl-30(n715) | G protein α-subunit | 3.7±1.0 | ns | - |
| 35 | C26G2.1p::DsRed2 | Control for Mb::YFP | 3.8±0.7 | ns | - |
| . | Genotype* . | Description . | Mean† . | P‡ . | P§ . |
|---|---|---|---|---|---|
| 1 | trIs30 (15°C) | Wild-type young adults | 3.8±1.1 | ns | - |
| 2 | trIs30 (20°C) | Wild-type young adults | 3.5±0.9 | - | - |
| 3 | trIs30 (25°C) | Wild-type young adults | 3.7±1.0 | ns | - |
| 4 | trIs30(ORNAi) | Wild-type young adults | 3.5±0.8 | ns | - |
| 5 | trIs30 (20°C) | Wild-type L1 hatchlings | 1.7±0.8¶ | <0.001 | - |
| 6 | sma-6(e1482) | Tgf-β receptor (small worms) | 3.4±0.8 | ns | - |
| 7 | lon-2(e678) | Glypican (long worms) | 3.7±1.2 | ns | - |
| 8 | dpy-5(e61)¶ | Collagen (short worms) | 3.6±0.7¶ | ns | - |
| 9 | dyp-10(e128) | Collagen (control for unc-104 null) | 3.6±0.9¶ | ns | - |
| 10 | act-1,2,3(st15) | Actin | 2.4±1.0¶ | <0.001 | <0.001 (5) |
| 11 | unc-60(su158) | ADF/cofilin (null) | 1.8±0.9 | <0.001 | ns (5) |
| 12 | unc-60(e723) | ADF/cofilin (hypomorph) | 1.8±0.8 | <0.001 | ns (5) |
| 13 | unc-60(s1307) | ADF/cofilin (hypermorph) | 3.3±1.0 | ns | <0.001 (11) |
| 14 | unc-60(r398) | ADF/cofilin (no severing) | 3.3±1.2 | ns | <0.001 (11) |
| 15 | unc-78(gk27) | Actin-interacting protein 1 (null) | 3.4±1.0 | ns | - |
| 16 | lev-11(TM1-RNAi) | Tropomyosin | 2.1±1.0 | <0.001 | ns (5) |
| 17 | lev-11(TM2-RNAi) | Tropomyosin | 2.2±1.1 | <0.001 | 0.038 (5) |
| 18 | act-1,2,3(st15); lev-11(TM1-RNAi) | - | 1.7±0.8¶ | <0.001 | ns (15)/ns (5) |
| 19 | unc-60(su158); lev-11(TM1-RNAi) | - | 1.4±0.6¶ | <0.001 | ns (10)/ns (5) |
| 20 | unc-54(e190) | Muscle myosin (MHC B) (null) | 2.4±1.1 | <0.001 | <0.001 (5) |
| 21 | unc-54(e190) | unc-54 L1 hatchlings (null) | 1.7±0.7¶ | <0.001 | ns (5) |
| 22 | unc-54(e1152) | Muscle myosin (MHC B) | 2.3±1.1 | <0.001 | - |
| 23 | unc-54(RNAi) | Muscle myosin (MHC B) | 2.3±1.2 | <0.001 | ns (20) |
| 24 | unc-15(e73)¶ | Paramyosin | 2.7±1.1 | <0.001 | - |
| 25 | unc-15(su2000)¶ | Paramyosin | 3.0±1.0 | <0.001 | - |
| 26 | unc-15(RNAi) | Paramyosin | 3.1±1.2 | <0.001 | 0.007 (24) |
| 27 | unc-15(e1402ts) (25°C)¶ | Paramyosin | 3.1±1.0 | 0.003 | - |
| 28 | unc-54(e190); lev-11(TM1-RNAi) | - | 2.1±0.9 | <0.001 | - |
| 29 | act-1,2,3(st15); unc-54(RNAi) | - | 2.9±0.9¶ | <0.001 | 0.037 (10) |
| 30 | unc-60(su158); unc-54(RNAi) | - | 1.6±0.8 | <0.001 | ns (11) |
| 31 | unc-104(e1265) | Kinesin | 1.7±1.2 | <0.001 | ns (5) |
| 32 | unc-104(rh43) | Kinesin | 1.3±1.0 | <0.001 | 0.011 (5) |
| 33 | unc-104(rh142) dyp-10(e128) | unc-104 null | 1.2±0.6¶ | <0.001 | <0.001 (5) |
| 34 | egl-30(n715) | G protein α-subunit | 3.7±1.0 | ns | - |
| 35 | C26G2.1p::DsRed2 | Control for Mb::YFP | 3.8±0.7 | ns | - |
All counts were made in the trIs30 background, except ¶,which were carried out with a him-4p::Mb::YFP extra-chromosomal array. All counts are from young adults raised at 20°C unless otherwise indicated.
Shown is the average number of muscle arms per BWM for the dorsal right quadrant, followed by the s.d. Only BWMs 9, 11, 13, 15, 17 and 19 were considered, except ¶, where BWMs 9, 11, 13 and 15 were considered. The arms of 15 muscles of each type were counted for each genotype, except for trIs30 at 20°C and trIs30 hatchlings, where 30 muscles of each type were counted.
These P values were derived from Mann-Whitney tests with respect to the trIs30 counts (row 2).
These P values were derived from Mann-Whitney tests with respect to the counts from the row indicated in brackets. ns, not significant(P>0.05). For those counts where only muscles 9-15 were considered, all statistical tests were carried out by comparing these counts to that of muscles 9-15 of the respective genotypes. The mean number of arms for each muscle is shown in Table S1 in the supplementary material.