The clonal relationship and location of neurons and glia that originate from the Wg domains was examined by using the FLP/FRT method to generate rare,small mitotic clones marked by UAS-CD8::GFP transgene expression. Glia were identified with anti-Repo antibody. For each specimen, clones were scored as labeling glia only (Glia), neurons only (neuron) or a mixed population of glia and neurons (glia and neurons) and the location of each clone was identified in accordance with the domain map shown in Fig. 4A. In total, 78 specimens were analyzed.