Average litter sizes of matings among different Tnfip6genotypes
| Mating pairs (Tnfip6 genotype) . | . | Number of pregnant females/number of matings . | Average size of litter per pregnancy . | |
|---|---|---|---|---|
| Female* . | Male . | . | . | |
| +/+ | +/+ | 11/12 | 7.20 | |
| +/+ | +/- | 14/14 | 6.05 | |
| +/+ | -/- | 6/7 | 6.29 | |
| +/- | +/+ | 6/6 | 6.50 | |
| +/- | +/- | 56/58 | 6.87 | |
| +/- | -/- | 9/10 | 7.00 | |
| -/-† | +/+ | 0/48 (16) | 0 | |
| -/-† | +/- | 0/31 (9) | 0 | |
| -/-† | -/- | 0/14 (9) | 0 | |
| -/-‡ | +/+ | 8/9 | 1.75 | |
| -/-§ | +/+ | 3/15 | 1.0 | |
| -/-¶(Tnfip6-Tg+) | +/+ | 5/9 | 4.0 | |
| Mating pairs (Tnfip6 genotype) . | . | Number of pregnant females/number of matings . | Average size of litter per pregnancy . | |
|---|---|---|---|---|
| Female* . | Male . | . | . | |
| +/+ | +/+ | 11/12 | 7.20 | |
| +/+ | +/- | 14/14 | 6.05 | |
| +/+ | -/- | 6/7 | 6.29 | |
| +/- | +/+ | 6/6 | 6.50 | |
| +/- | +/- | 56/58 | 6.87 | |
| +/- | -/- | 9/10 | 7.00 | |
| -/-† | +/+ | 0/48 (16) | 0 | |
| -/-† | +/- | 0/31 (9) | 0 | |
| -/-† | -/- | 0/14 (9) | 0 | |
| -/-‡ | +/+ | 8/9 | 1.75 | |
| -/-§ | +/+ | 3/15 | 1.0 | |
| -/-¶(Tnfip6-Tg+) | +/+ | 5/9 | 4.0 | |
All females were offspring of Tnfip6+/- parents or wild-type mothers and Tnfip6-/- fathers. In all cases, one female mouse was mated with a male in a 3-day rotation cycle until the female became pregnant.
For Tnfip6-/- females, the numbers in parentheses indicate the number of females. These 34 females were mated repeatedly with stud males in the `standard' 3-day rotation cycle. Some of these stud males were selected from other wild-type colonies.
Tnfip6-/- females were superovulated and the hCG co-injected with 100 μg of mouse recTNFIP6.
Tnfip6-/- females were superovulated without adding exogenous TNFIP6; only 3 females became pregnant and delivered one baby of each.
Tnfip6-/- females without superovulation, but carrying the Tnfip6 transgene, were mated with wild-type stud males.