Primer sequences used for genotyping and RT-PCR
| Primer name . | Location . | Sequence (5′→3′) . | Product size (bp)* . |
|---|---|---|---|
| Neo-13Fw | Neo | GGATCGGCCATTGAACAAG | 600 |
| Neo-14Rev | Neo | CACCATGATATTCGGCAAGC | |
| Tnfip6-Fw1 | Neo | GGGTGGGATTAGATAAATGC | 441 |
| Tnfip6-Rev1 | Intron-1 | TCACTCCTTCCATTCAGTCC | |
| Tnfip6-Fw2 | Exon-1 | TGCCAATGCAAATGAGTCAGAGG | 356 |
| Tnfip6-Rev2 | Intron-1 | GGATCGATATTCCCAACATGAAGC | 2,156 |
| Tnfip6-Fw3 | Exon-1 | CGTCCTCCTTTGCTTATGC | 490 |
| Tnfip6-Rev3 | Exon-4 | AATGTGCCAGTAGCAGACC |
| Primer name . | Location . | Sequence (5′→3′) . | Product size (bp)* . |
|---|---|---|---|
| Neo-13Fw | Neo | GGATCGGCCATTGAACAAG | 600 |
| Neo-14Rev | Neo | CACCATGATATTCGGCAAGC | |
| Tnfip6-Fw1 | Neo | GGGTGGGATTAGATAAATGC | 441 |
| Tnfip6-Rev1 | Intron-1 | TCACTCCTTCCATTCAGTCC | |
| Tnfip6-Fw2 | Exon-1 | TGCCAATGCAAATGAGTCAGAGG | 356 |
| Tnfip6-Rev2 | Intron-1 | GGATCGATATTCCCAACATGAAGC | 2,156 |
| Tnfip6-Fw3 | Exon-1 | CGTCCTCCTTTGCTTATGC | 490 |
| Tnfip6-Rev3 | Exon-4 | AATGTGCCAGTAGCAGACC |
For the corresponding amplification results see Fig. 1C and D.