FRL-1MOs specifically induced head defect phenotypes
| . | . | Phenotype (%) . | . | |
|---|---|---|---|---|
| . | Numbers . | Head defect (without eyes) . | Normal head . | |
| FRL-1-4-misMO 15 ng | 50 | 0 | 100 | |
| FRL-1MO 15 ng | 50 | 90 | 10 | |
| FRL-1MO 15 ng (veg) | 50 | 0 | 100 | |
| FRL-1MO 15 ng +FRL-1Δ5′UTR 20 pg | 50 | 18 | 82 | |
| FRL-1MO 15 ng +FRL-1ΔEGF 20 pg | 50 | 96 | 4 | |
| FRL-1MO 15 ng +FRL-1ΔCFC 20 pg | 51 | 98 | 2 | |
| FRL-1MO 15 ng +oep 20 pg | 52 | 31 | 69 | |
| FRL-1MO 15 ng +cripto 20 pg | 52 | 29 | 71 | |
| FRL-1MO 15 ng +chordin 20 pg | 50 | 94 | 6 | |
| FRL-1MO 15ng +noggin 20 pg | 50 | 88 | 12 | |
| FRL-1MO 15 ng +CA-MAPKK 2 ng | 52 | 21 | 79 | |
| FRL-1-4-misMO 40 ng | 50 | 2 | 98 | |
| FRL-1MO-second 40 ng | 50 | 88 | 12 | |
| FRL-1MO-second 40 ng +FRL-1Δ5′UTR 20 pg | 51 | 22 | 78 | |
| . | . | Phenotype (%) . | . | |
|---|---|---|---|---|
| . | Numbers . | Head defect (without eyes) . | Normal head . | |
| FRL-1-4-misMO 15 ng | 50 | 0 | 100 | |
| FRL-1MO 15 ng | 50 | 90 | 10 | |
| FRL-1MO 15 ng (veg) | 50 | 0 | 100 | |
| FRL-1MO 15 ng +FRL-1Δ5′UTR 20 pg | 50 | 18 | 82 | |
| FRL-1MO 15 ng +FRL-1ΔEGF 20 pg | 50 | 96 | 4 | |
| FRL-1MO 15 ng +FRL-1ΔCFC 20 pg | 51 | 98 | 2 | |
| FRL-1MO 15 ng +oep 20 pg | 52 | 31 | 69 | |
| FRL-1MO 15 ng +cripto 20 pg | 52 | 29 | 71 | |
| FRL-1MO 15 ng +chordin 20 pg | 50 | 94 | 6 | |
| FRL-1MO 15ng +noggin 20 pg | 50 | 88 | 12 | |
| FRL-1MO 15 ng +CA-MAPKK 2 ng | 52 | 21 | 79 | |
| FRL-1-4-misMO 40 ng | 50 | 2 | 98 | |
| FRL-1MO-second 40 ng | 50 | 88 | 12 | |
| FRL-1MO-second 40 ng +FRL-1Δ5′UTR 20 pg | 51 | 22 | 78 | |
Note: MOs and mRNAs were animally injected into each blastomere of 8-cell-stage embryos, except where denoted by (veg) which indicates vegetal injection.