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Table 1.

Effects of SCR base substitutions on reporter mRNA translation

Reporter mRNATotal gonads injected% gonads with strong β-gal expression in embryos*% gonads with weak β-gal expression in embryos *% embryos with anterior localization (n)
lacglp(wt) 89 26 93 (27) 
lacglp(M7) 48 35 27 0 (20) 
lacglp(M8) 29 41 
lacglp(LS1) 110 20 17 0 (24) 
lacglp(LS2) 93 
lacglp(LS3) 76 13 
lacglp(LS4) 48 13 
lacglp(LS5) 39 21 
lacglp(LS1LS2) 69 41 16 3 (30) 
Reporter mRNATotal gonads injected% gonads with strong β-gal expression in embryos*% gonads with weak β-gal expression in embryos *% embryos with anterior localization (n)
lacglp(wt) 89 26 93 (27) 
lacglp(M7) 48 35 27 0 (20) 
lacglp(M8) 29 41 
lacglp(LS1) 110 20 17 0 (24) 
lacglp(LS2) 93 
lacglp(LS3) 76 13 
lacglp(LS4) 48 13 
lacglp(LS5) 39 21 
lacglp(LS1LS2) 69 41 16 3 (30) 
*

Staining in embryos was scored as `strong' if β-gal was easily detected at 50× magnification, and `weak' if staining was detected only at 400×. All mRNAs produced strong expression when injected into somatic cells.

Four- to 16-cell embryos in which staining and cell identity could be unambiguously determined were scored as `anterior localized' if staining was strong in AB-derived cells and undetectable in at least two (4- to 6-cell embryos) or four (8- to 16-cell embryos) P1-derived cells. - indicates that localization β-gal could not be scored because of low or absent staining.

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