Optimal primer pairs for Real Time PCR
| Gene . | Accession No. . | Sense . | Antisense . | Location . | Size . |
|---|---|---|---|---|---|
| GlcNAcT-I | AF000416 | CCTTGTGGCCCTGCCA | GTTACATCAAAACCATGCTGGACT | 1782-1844 | 62 |
| HS2ST | AB024568 | ATTATGATGCCGCCCAAGTT | AAGAGCATCGCCACCGC | 370-431 | 61 |
| CS/DS2ST | AB020316 | GTCAGCAAGCCCCCCT | TCGATTGGCTCCTCGGTTT | 1205-1272 | 67 |
| HS6ST1 | NM_004807 | GCGTGGAGGTGGATGAAGAC | TGCATGTCCAGGTCGTTGAG | 1085-1145 | 60 |
| HS6ST2 | AB024565 | CCTGGTGCGCAACATCC | TGCATTTCTTCTGCCCCAC | 298-359 | 61 |
| NDST1 | NM_001543 | TCCTGGAGTCCAGCCGC | CGTGGGCATGTCACCCTT | 731-795 | 64 |
| NDST2 | NM_003635 | AGACCGGTACTGCGTGGAGT | GGCTGTGCTGCTGGGCT | 498-562 | 64 |
| Epimerase | AF003927 | CCATCTATGACCTCCGGCAC | AGTCCCAGCGGGCCAG | 1226-1285 | 59 |
| Gene . | Accession No. . | Sense . | Antisense . | Location . | Size . |
|---|---|---|---|---|---|
| GlcNAcT-I | AF000416 | CCTTGTGGCCCTGCCA | GTTACATCAAAACCATGCTGGACT | 1782-1844 | 62 |
| HS2ST | AB024568 | ATTATGATGCCGCCCAAGTT | AAGAGCATCGCCACCGC | 370-431 | 61 |
| CS/DS2ST | AB020316 | GTCAGCAAGCCCCCCT | TCGATTGGCTCCTCGGTTT | 1205-1272 | 67 |
| HS6ST1 | NM_004807 | GCGTGGAGGTGGATGAAGAC | TGCATGTCCAGGTCGTTGAG | 1085-1145 | 60 |
| HS6ST2 | AB024565 | CCTGGTGCGCAACATCC | TGCATTTCTTCTGCCCCAC | 298-359 | 61 |
| NDST1 | NM_001543 | TCCTGGAGTCCAGCCGC | CGTGGGCATGTCACCCTT | 731-795 | 64 |
| NDST2 | NM_003635 | AGACCGGTACTGCGTGGAGT | GGCTGTGCTGCTGGGCT | 498-562 | 64 |
| Epimerase | AF003927 | CCATCTATGACCTCCGGCAC | AGTCCCAGCGGGCCAG | 1226-1285 | 59 |