... osteoblast differentiation. We conclude that BRD4 controls facial bone development through osteoblast enhancer regulation of the RUNX2 transcriptional program. Introduction Cornelia de Lange syndrome (CdLS) is a multi-organ congenital disorder featuring facial dysmorphism, growth retardation, developmental...

... abnormalities. Bulk and single-cell RNA-seq profiling reveal that loss of both Tfap2 family members dysregulates numerous midface GRN components involved in midface morphogenesis, patterning, and differentiation. Notably, Alx1/3/4 ( Alx ) transcript levels are reduced, while ChIP-seq analyses suggest TFAP2...

...), or complete on one side (2 out of 8). Histological analyses revealed that the uterine body and vagina displayed a columnar epithelium (Fig. 5I) and a stratified squamous epithelium (Fig. 5J), as anticipated for these female organs. In addition, Nr5a1SC males displayed 40% lighter seminal vesicles [4.71 ± 1.66...

...Martina Cerise; Vítor da Silveira Falavigna; Gabriel Rodríguez-Maroto; Antoine Signol; Edouard Severing; He Gao; Annabel van Driel; Coral Vincent; Sandra Wilkens; Francesca Romana Iacobini; Pau Formosa-Jordan; Alice Pajoro; George Coupland ABSTRACT Plant organ primordia develop successively...

... progression occurs by rapid and concerted transcriptome-wide switching from one gene expression state to the next ( Antolovic et al., 2019 ; Artegiani et al., 2017 ; Giri et al., 2022 ; Jang et al., 2017 ; Moris et al., 2016 ; Nelms and Walbot, 2019 ; Rukhlenko et al., 2022 ; Saez et al., 2022...

.... Am Heart J 154, 596-602. Ervasti, J. M. (2003). Costameres: the Achilles' heel of Herculean muscle. J Biol Chem 278, 13591-4. Ervasti, J. M. and Campbell, K. P. (1991). Membrane organization of the dystrophinglycoprotein complex. Cell 66, 1121-31. Ervasti, J. M., Kahl, S. D. and Campbell, K. P. (1991...

... cardiomyocytes (CMs) instead of a heterogeneous organ (see Materials and Methods) ( Lian et al., 2013 ). The genome-wide H2Bub1 profile and corresponding transcriptional changes during CM differentiation were analyzed by ChIP-seq of H2Bub1 and bulk RNA-seq at five stages of CM differentiation: iPSCs, mesoderm (M...

... to form the three mature rRNAs (28S, 18S, and 5.8S) ( Henras et al., 2015 ; Schöfer and Weipoltshammer, 2018 ). Active and inactive rDNA copies are organized by different chromatin conformations, epigenetic marks, topological organization and nucleolar location ( Németh and Längst, 2011 ; Grummt...

... ­– the ability to perceive weak electric fields – is found almost exclusively in aquatic or semi-aquatic species and can be either active or passive. While weakly electric fish (Gymnotiformes and Mormyriformes) generate electric discharges (electric organ discharges, EODs) with specialized electric organs...

.... To achieve this, each dividing cell builds a mitotic spindle that results from a spectacular rearrangement of microtubules, allowing proper alignment of chromosomes during metaphase and their equal separation during anaphase ( Prosser and Pelletier, 2017 ). In polarised epithelia of multicellular organisms...

... genes for the development of compact cardiomyocytes including myocardin ( Myocd ) and serum response factor ( SRF ). In addition, ChIP-sequencing and co-immunoprecipitation analyses revealed that Sall4 and Myocd form a transcriptional complex with SRF and directly bind to the upstream regulatory regions...

..., and the impact of a severe injury can extend far beyond the injured organ. Disruption of homeostasis in one organ can elicit organism-wide cellular, molecular and physiological changes in animals. In some cases, distant organs are activated as part of the compensatory mechanism for changes in functionality...

... intensity, indicating that Zld-binding contributes directly to stripe activation. However, reductions caused by Zld site mutations were consistently weaker than those caused by removing Zld activity in trans , suggesting that Zld also plays indirect roles. We show that one of the most important indirect...

... and prevent cardiac rupture, however, the formation of a rigid scar impairs both mechanical and electrical coupling of the tissue, leading to worsened heart function. While fibrosis occurs in many organ systems in various states of disease (Wynn, 2004), mechanisms are not always conserved, and thus need...

... I have identified; you may have others (or different ones), but these are mine. These problems concern those who are employed by institutions, as opposed to students and other ‘trainees’ (yes, there are problems there as well, and while I will touch on these, they deserve another discussion. Indeed...

.... , Haderspeck , J. C. , Bolz , S. , Rogal , J. , Chuchuy , J. , Nikolova , M. , Cora , V. , Antkowiak , L. , Haq , W. et al. ( 2019 ). Merging organoid and organ-on-a-chip technology to generate complex multi-layer tissue models in a human retina-on-a-chip platform . ELife...

... development to a level previously only attained with nonmammalian organisms. Building on data resources such as the one reported here, mechanistic insights will continue to require perturbation experiments, with the important proviso that carefully chosen experimental perturbations have the added benefit...

... repository under accession number GSE73867 ( O'Brien et al., 2016 ). Pre-processed SIX1 ChIP-seq data from Wilms tumors were kindly shared by Dr Manfred Gessler ( Wegert et al., 2015 ) in the form of bigWig files from two SIX1 wild-type ChIP-seq replicates (one replicate from each of the two tumors...

... analysis found that many binding motifs were unique to one or the other isoform, only one out of the top six motifs were common between the isoforms ( Figs 6 , 7 ). Next, we asked whether the SIN3 binding motifs are shared with any transcription factor binding motifs. To this end, we used Tomtom...

... that blocking PI3K activation prevents the loss of apical polarity ( Tenvooren et al., 2019 ). We attribute the increase in MP during glandular differentiation to the progressive organization of the basoapical polarity axis. In vitro , acinus morphogenesis typically starts from one cell and results from...