... and Lactobacillus plantarum , but were repelled by Acetobacter malorum in behavioral assays, indicating that an olfactory mechanism is involved in these preference behaviors. While the attraction to yeast was governed by olfactory sensory neurons expressing the odorant co-receptor Orco, the observed behaviors...

...Keren Aviezer-Hagai; Vered Padler-Karavani; Nathan Nelson SUMMARY V-ATPase null mutants in yeast have a distinct, conditionally lethal phenotype that can be obtained through disruption of any one of its subunits. This enables supplementation of this mutant with the relevant subunit tagged...

...Natalie Perzov; Vered Padler-Karavani; Hannah Nelson; Nathan Nelson SUMMARY Subunit a of V-ATPase in the yeast Saccharomyces cerevisiae , in contrast to its other subunits, is encoded by two genes VPH1 and STV1 . While disruption of any other gene encoding the V-ATPase subunits results in growth...

... about the transport mechanism of metal ions by eukaryotic cells. Some insight has been gained from studies of the mammalian transporter DCT1 and the yeast transporter Smf1p by following the uptake of various metal ions and from electrophysiological experiments using Xenopus laevis oocytes injected...

...Tamar R. Grossman; Juan M. Luque; Nathan Nelson ABSTRACT A family of genes encoding membrane proteins with a unique structure has been identified in DNA and cDNA clones of various eukaryotes ranging from yeast to human. The nucleotide sequences of three novel cDNAs from Drosophila melanogaster...

...Anthony Ambesi; Manuel Miranda; Valery V. Petrov; Carolyn W. Slayman ABSTRACT One of the most abundant proteins in the yeast plasma membrane is the P-type H + -ATPase that pumps protons out of the cell, supplying the driving force for a wide array of H + -dependent cotransporters. The ATPase...

...Laurie A. Graham; Ben Powell; Tom H. Stevens ABSTRACT The proton-translocating ATPase (H + -ATPase) found on the membrane of the yeast vacuole is the best characterized member of the V-type ATPase family. Biochemical and genetic screens have led to the identification of 14 genes, the majority...

...Michael Forgac ABSTRACT The vacuolar H + -ATPases (or V-ATPases) are a family of ATP-dependent proton pumps that carry out acidification of intracellular compartments in eukaryotic cells. This review is focused on our work on the V-ATPases of clathrin-coated vesicles and yeast vacuoles. The coated...

... force for numerous secondary transport processes. The enzyme is also vital for the proper functioning of endosomes and the Golgi apparatus. In contrast to yeast vacuoles, which maintain an internal pH of approximately 5.5, it is believed that the vacuoles of lemon fruit may have a pH as low as 2...

...Patricia M. Kane; Karlett J. Parra ABSTRACT The yeast vacuolar H + -ATPase (V-ATPase) consists of a complex of peripheral subunits containing the ATP binding sites, termed the V 1 sector, attached to a complex of membrane subunits containing the proton pore, termed the V o sector. Interaction...

...Cynthia Church; Robert O. Poyton ABSTRACT Previous studies have reported that mitochondrial morphology and volume in yeast cells are linked to cellular respiratory capacity. These studies revealed that mitochondrial morphology in glucose-repressed or anaerobically grown cells, which lack or have...

...-encoded subunits are encoded by multigene families. The isoforms produced by these multigene families are tissue-specific and/or developmentally regulated in mammals and environmentally regulated in lower eukaryotes. Isoforms for one of the subunits, V, in the yeast Saccharomyces cerevisiae and one...

...Kurt E. Kwast; Patricia V. Burke; Robert O. Poyton ABSTRACT The budding yeast Saccharomyces cerevisiae is a facultative aerobe that responds to changes in oxygen availability (and carbon source) by initiating a biochemically complex program that ensures that energy demands are met under two...

...Frantisek Supek; Lubica Supekova; Hannah Nelson; Nathan Nelson ABSTRACT A novel Saccharomyces cerevisiae mutant, unable to grow in the presence of 12.5 mmol l −1 EGTA, was isolated. The phenotype of the mutant is caused by a single amino acid change (Gly149 to Arg) in the essential yeast cell...

... subunits were no longer associated with vacuolar membranes yet were present at wild-type levels in yeast whole-cell extracts. The VPH1 gene was cloned by screening a λgtl 1 expression library with antibodies directed against a 95 kDa vacuolar integral membrane protein and independently cloned...

...Daniel J. Klionsky; Hannah Nelson; Nathan Nelson; Debbie S. Yaver ABSTRACT The vacuolar ATPase of the yeast Saccharomyces cerevisiae acidifies the vacuolar lumen and generates an electrochemical gradient across the vacuole membrane. We have investigated the role of compartment acidification...