... between groups of flasks collected at different time points. Fig. 5. Temporal change in the resistive properties and paracellular permeability of primary cultured rainbow trout gill cells. (A) Transepithelial resistance (TER) and (B) [ 3 H]PEG-400 flux across primary cultured single-seeded...

... voltage cell input resistance transepithelial voltage transepithelial resistance In the past two decades, our laboratory has investigated ion secretion in Malpighian tubules of the female yellow fever mosquito, Aedes aegypti (Beyenbach, 1995 , 2001 ; Beyenbach and Petzel, 1987 ). Our...

... and mitochondria-rich cells in the approximate proportion in which they occur in vivo and to exhibit diffusive fluxes of Na + and Cl – similar to in vivo values, but does not exhibit active apical-to-basolateral transport of Na + . Transepithelial resistance and paracellular permeability are also known to increase...

... with Leibovitz’s L-15 medium on both surfaces (symmetrical conditions), transepithelial resistance (TER) stabilized at values as high as 34 kΩ cm 2 , indicative of electrically ‘tight’ epithelia. The density of MR cells, the surface area of their clusters and transepithelial potential (TEP; up to +8 mV basolateral...

... foetal bovine serum and glutamine) are trypsinized and the cells seeded onto the inserts. After 6 days of growth with L-15 medium on both surfaces (approximately isotonic to trout plasma), the cells form a tight epithelium as judged from a progressive rise in transepithelial resistance which reaches...