...Dennis Kolosov; Scott P. Kelly ABSTRACT Cortisol-induced epithelial tightening of a primary cultured rainbow trout gill epithelium model occurs in association with reduced paracellular permeability and increased abundance of select barrier-forming tight junction (TJ) proteins. Corticosteroid...

...William S. Marshall; Jason P. Breves; Ellen M. Doohan; Christian K. Tipsmark; Scott P. Kelly; George N. Robertson; Patricia M. Schulte ABSTRACT To provide insight into claudin (Cldn) tight junction (TJ) protein contributions to branchial salt secretion in marine teleost fishes, this study examined...

... is modified to allow Na + secretion in hypersaline environments. Mummichogs ( Fundulus heteroclitus ) acclimated to hypersaline [2× seawater (2SW), 64‰] for 30 days developed invasive projections of accessory cells with an increased area of tight junctions, detected by punctate distribution of CFTR (cystic...

...-10d and -10e did not. This study provides direct evidence that the gill epithelium contains salinity-responsive tight junction proteins that are abundant primarily in ionocytes. These same proteins also appear to play a role in the osmoregulatory physiology of the epidermis. * Author...

... absorptive demand. We measured absorption of l -arabinose, intestinal size and gene expression of several tight junction proteins (claudin-2, claudin-4, claudin-15 and ZO-1) at three time points: pre-exposure, and after 1 day and 2 weeks of cold exposure. Cold exposure increased food intake by 62% after 2...

... tissues in which absorption of chloride against an electrochemical gradient was observed, requiring a tight paracellular barrier to prevent back-leakage. However, little is known about ruminal barrier function, despite the considerable implications for ruminant health. The tight junction proteins...

... are largely unknown, but tight junctions that regulate the paracellular pathway are prime candidates in this adaptational process. Using differential display polymerase chain reaction we found a set of 17 differentially regulated genes in trout pavement cells that had been exposed to freshwater apically...

...Helen Chasiotis; Scott P. Kelly SUMMARY To investigate the response of the tight junction (TJ) protein occludin to environmental change in an anuran amphibian, we examined occludin tissue distribution, immunolocalization and alterations in mRNA expression in African clawed frogs ( Xenopus laevis...

...Helen Chasiotis; Scott P. Kelly SUMMARY Tight junctions (TJs) are an integral component of models illustrating ion transport mechanisms across fish epithelia; however, little is known about TJ proteins in fishes. Using immunohistochemical methods and Western blot analysis, we examined...

...+paracellular) were similar between the two species. The greater absorption of these probes in pigeons could not be explained by mediated uptake of the putative paracellular probes, or by increased nominal surface area, increased villus area or increased number of tight junctions. Rats and pigeons had...

... are not required for signaling or mediating the effects of leucokinin in Malpighian tubules of Aedes aegypti . The results further support previous observations that principal cells signal the effects of leucokinin to increase the Cl - conductance of the paracellular pathway through septate (or tight) junctions...