... channel. J. Gen. Physiol. 124 , 703 -718. * Author for correspondence (e-mail: [email protected] ) 2 9 2008 © The Company of Biologists Limited 2008 2008 site-directed mutagenesis ion channel gating electrophysiology Voltage-gated potassium channels...

... jShak1 jShak2 site-directed mutagenesis S4 segment voltage sensing charge evolution activation inactivation gating valence threshold The Shaker sub-family of K + channel genes appears to be ubiquitous in both invertebrates ( Papazian et al . 1987 ; Kamb et al . 1987 ; Tempel...

...-directed fluorescence spectroscopy to study structure–function relationships in the permease. ©The Company of Biologists Limited 1994 bioenergetics transport site-directed mutagenesis site-directed spectroscopy membrane proteins cys-scanning mutagenesis An unsolved basic biochemical...

... functions are properly articulated in relation to other aspects of cell function. ©The Company of Biologists Limited 1994 anion exchange cysteine scanning mutagenesis site-directed mutagenesis sulfhydryl reagents substrate translocation pathway membrane carrier indirect proton pumps UhpT...

... 1994 neurotransmitter transporters sodium cotransport purification molecular cloning site-directed mutagenesis The deduced amino acid sequences of these proteins reveal 30–65% identity between different members of the family. On the basis of these differences in homology, the family...