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Keywords: short-circuit current
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Journal Articles
J Exp Biol (2004) 207 (26): 4623–4631.
Published: 15 December 2004
... in the posterior gills was nearly fourfold that of anterior gills, while V-ATPase and F-ATPase activities did not differ. A negative short-circuit current ( I sc ) was measured using the distal side of split, posterior gill lamellae, mounted in a modified Ussing chamber and perfused symmetrically with identical...
Journal Articles
J Exp Biol (2003) 206 (6): 1017–1023.
Published: 15 March 2003
... positive) was measured. After voltage-clamping, the negative short-circuit current( I sc ) amounted to -142±21 μA cm -2 at a conductance (G te ) of 44±5 mS cm -2 . Substitution of either chloride (by nitrate) or sodium (by choline) on both sides of split gill lamellae significantly reduced I sc (by 70-80...
Journal Articles
J Exp Biol (2003) 206 (3): 619–626.
Published: 1 February 2003
... AMP gills hyperosmoregulation perfused gills Na + /K + -ATPase short-circuit current split gill lamellae transepithelial conductance transepithelial voltage Table 2. Mean values of short-circuit current ( I sc )and transepithelial conductance ( G te ) before and after osmotic...
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J Exp Biol (2001) 204 (8): 1509–1517.
Published: 15 April 2001
... integument, none of them mimicked the effect of an experimental rise in intracellular cyclic AMP level. The invertebrate peptides conopressin and angiotensin II amide inhibited short-circuit-current-( I sc ) and amiloride-sensitive Na + transport ( I amil ), although to slightly different degrees...
Journal Articles
J Exp Biol (1998) 201 (22): 3067–3074.
Published: 1 November 1998
...Kyeong-Yeoll Lee; Frank M. Horodyski; M. E. Chamberlin ABSTRACT Short-circuit current ( I sc ) across the posterior midgut of day 2 fifth-instar tobacco hornworms ( Manduca sexta ) is inhibited by Manduca allatotropin (Mas-AT) and two Manduca FLRFamides (F7G and F7D). Another FLRFamide, F10...
Journal Articles
J Exp Biol (1998) 201 (11): 1753–1762.
Published: 1 June 1998
... fever mosquito Aedes aegypti . A low concentration of CCRF-DP (10 −9 mol l −1 ) caused a small but insignificant increase in transepithelial secretion of NaCl and fluid, but significantly reduced transepithelial voltage and resistance without a change in short-circuit current, pointing...
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J Exp Biol (1995) 198 (3): 767–774.
Published: 1 March 1995
... generation, which were markedly reduced in the presence of bafilomycin. In addition to these biochemical studies, we mounted split lamellae of posterior gills in an Ussing-type chamber and measured the negative short-circuit current ( I sc ), which was shown to reflect active, electrogenic, Na + -independent...
Journal Articles
J Exp Biol (1994) 197 (1): 179–200.
Published: 1 December 1994
...K. Schirmanns; W. Zeiske ABSTRACT The K + -secreting larval midgut of Manduca sexta in vitro was voltage-or current-clamped. In contrast to Tl + , NH4 + and Na + , both Rb + and K + generated a short-circuit current, although with different saturation kinetics. The dependence of the short-circuit...
Journal Articles
J Exp Biol (1994) 188 (1): 191–204.
Published: 1 March 1994
...K. Schirmanns; W. Zeiske ABSTRACT Active K + secretion in isolated posterior midguts of Manduca sexta was studied by measuring the short-circuit current. One aim of this study was to verify the postulate from biochemical reports that the cooperative apical arrangement of a vacuolar-type H + -ATPase...
Journal Articles
J Exp Biol (1994) 188 (1): 159–174.
Published: 1 March 1994
... and negative Cl − -dependent short-circuit currents ( I Na, I Cl), respectively. Both currents were characterized before and after treatment of the tissue with theophylline or dibutyryl cyclic AMP. Both drugs increased I Na and I Cl. A simple circuit analysis showed that I Na stimulation reflected a marked...
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Journal Articles
J Exp Biol (1990) 150 (1): 425–442.
Published: 1 May 1990
... and sugars, it was shown that all three sections actively secrete K + and Cl − and absorb Na + . The anterior section maintained the highest short-circuit current ( I sc ), transepithelial potential difference (PD) and net K + secretion. The middle section had the lowest I sc , PD and K + secretion...
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Journal Articles
J Exp Biol (1988) 135 (1): 25–38.
Published: 1 March 1988
... in these studies (and most other midgut studies) are nominally sodium-free. Also, a sodium-stimulated, ouabain-inhibited ATPase activity is not detectable in the midgut ( Jungreis & Vaughn, 1977 ). Furthermore, ouabain is without effect on the short-circuit current ( Haskell, Clemmons & Harvey, 1965...