... of Biologists Ltd 2014 V-ATPase Acid/base cAMP Carbonic anhydrase pH sensing Proton pump Cyclic adenosine monophosphate (cAMP) is the signaling molecule of one of the most versatile and evolutionarily conserved signaling pathways. cAMP is produced by adenylyl cyclase enzymes that use ATP...

... as an index of anion exchange and thus Cl – absorption via this pathway. Similar involvement of proton pump in Cl – uptake via anion exchange was previously demonstrated for the freshwater fish gill and amphibian skin ( Fenwick et al.,1999 ; Jensen et al.,2002 ; Boisen et al.,2003 ; Jensen et al.,2003...

... of Epidemiology and Statistics, University of Florida,Gainesville, FL 32610, USA † Present address: Department of Physiology and Biophysics, Rosalind Franklin School of Medicine, North Chicago, IL 60064, USA 17 12 2007 © The Company of Biologists Limited 2008 2008 proton pump H + V...

... but also decreased steady-state mRNA expression of trout H + /K + -ATPase (ATP4A, the proton pump) as well as Na + /bicarbonate cotransporter (NBC), and had no effect on gastrin-like mRNA and somastostatin (SST) mRNA abundance. Gastrin-like mRNA and SST-2 mRNA were equally distributed between corpus...

... next to the V-type H + ATPase concentrates neurotransmitters in synaptic vesicles. First found in association with endosomal membranes, the V-type H + ATPase is now also found in increasing examples of plasma membranes where the proton pump energizes transport across cell membranes and entire epithelia...

... 11 2003 © The Company of Biologists Limited 2004 2004 ammonia excretion permeability cholesterol fatty acid gill H + -ATPase lipid membrane membrane fluidity mudskipper Periophthalmodon schlosseri phospholipid proton pump skin The giant mudskipper...

... and exocytosis. Their lumina are acidic, with pH ranging from 4.5 to 6.5. This article describes recent studies on these animal cell organelles focusing on (1) the primary proton pump (vacuolar-type H + -ATPase) and (2) the functions of the organelle luminal acidity. We also discuss similarities and differences...

... reticulum; (2) mutants in which the conformational equilibrium has been shifted from the E 2 state, characterized by high affinity for vanadate, to the E 1 state, characterized by high affinity for ATP; and (3) mutants with altered coupling between ATP hydrolysis and proton pumping. Although much remains...

... of Biologists 2000 membrane transport protein crystallization P-type ATPase proton pump structure conformational change molecular dynamics Ion-translocating ATPases are essential cellular energy converters that transduce the chemical energy of ATP hydrolysis into transmembrane ionic...

...Dennis Brown; Sylvie Breton ABSTRACT Many vertebrate transporting epithelia contain characteristic ‘mitochondria-rich’ cells that express high levels of a vacuolar proton-pumping ATPase (H + V-ATPase) on their plasma membrane and on intracellular vesicles. In the kidney cortex, A-cells and B-cells...

... apparent 30 min after the removal of bafilomycin. These data provide circumstantial evidence for previous suggestions that Na + uptake in freshwater fish is associated with a proton-motive force created by a proton pump and indirect evidence for the major significance of this mechanism in the branchial...

... Limited 1997 H + -translocating vacuolar-type ATPase H + V-ATPase proton pump regulation The term ‘cellular homeostasis’ summarizes the constant composition of cellular components. Thus, characteristic values of cell [K + ] are high, cell [Na + ] and [Mg 2+ ] are low and cell [Ca 2...

...Yoshinori Moriyama ABSTRACT Many organelles are energized by proton pumps: mitochondria form an inside-negative membrane potential by means of the respiratory chain and endomembrane structures, such as lysosomes and synaptic vesicles, establish an internal acidic pH by means of a vacuolar-type H...

... different subunits of 70, 60,44,42, 36, 32, 29, 16, 13 and 12 × 10 3 M r . This set of ten polypeptides is sufficient to couple ATP hydrolysis to proton pumping after reconstitution of the ATPase into liposomes. Unlike some animal V-ATPases, the purified and reconstituted V-ATPase from oat is directly...

... + -ATPase urinary acidification proton pump The kidney of vertebrates regulates acid base balance by reabsorption of filtered bicarbonate and by generating sufficient bicarbonate to titrate the acid that is produced during cellular metabolism. It is now accepted that this process is mediated by H...