... of goldfish. Then, the ghrelin regulation of clock gene expression in the goldfish liver was studied. Finally, the possible involvement of the phospholipase C/protein kinase C (PLC/PKC) and adenylate cyclase/protein kinase A (AC/PKA) intracellular signalling pathways was investigated. Ghrelin receptor...

..., membrane association of the V 1 subunits C and D increases, consistent with the assembly of the holoenzyme. The protein kinase A inhibitor H-89 abolishes all cAMP-induced effects, consistent with protein kinase A (PKA) being involved in V-ATPase activation. Metabolic inhibition induced by KCN, azide...

...-activated protein kinase (PKA). At the level of the sarcomere, it is typically the phosphorylation of cardiac myosin binding protein C (cMyBP-C) and cardiac troponin I (cTnI) that is responsible for the change in the kinetics of contraction and relaxation. Trout cTnI (ScTnI) lacks two critical PKA targets...

...–2.0 mmol l –1 ), followed by theophylline (0.5–2.0 mmol l –1 ) and caffeine(1–2.0 mmol l –1 ). The protein kinase A (PKA) inhibitor H89 stimulated oocyte maturation in a concentration-dependent manner. However,when co-incubated with 2-OHE 2 for 24 h it produced a biphasic effect: low concentrations...

...Armin Kurtz; Charlotte Wagner ABSTRACT Renin secretion at the level of renal juxtaglomerular cells appears to be controlled mainly by classic second messengers such as Ca 2+ , cyclic AMP and cyclic GMP, which in turn exert their effects through oppositely acting protein kinases and probably also...

... oocytes and then use electrophysiological methods to measure (i) the kinetics of Na + /glucose cotransport, (ii) the number of cotransporters in the plasma membrane, and (iii) the net rate of exo- and endocytosis before and after activation of protein kinases. To evaluate the role of cotransporter...

... is followed by a desensitisation of the transport system itself. Cloning and expression of βNHE have provided significant information about Na + /H + activation, in particular that activation by cyclic AMP is directly dependent upon the presence of two protein kinase A consensus sites in the cytoplasmic tail...