... by the activity of facilitative glucose transporters (GLUTs) and their localisation to the plasma membrane (PM). In this study, we determined the relative protein abundance of GLUT1, GLUT2, GLUT3 and GLUT5 via immunoblot using custom-designed antibodies in whole-tissue homogenates and PM fractions of flight...

...Marcin Czarnoleski; Brandon S. Cooper; Justyna Kierat; Michael J. Angilletta, Jr SUMMARY Although plasma membranes benefit cells by regulating the flux of materials to and from the environment, these membranes cost energy to maintain. Because smaller cells provide relatively more membrane area...

... next to the V-type H + ATPase concentrates neurotransmitters in synaptic vesicles. First found in association with endosomal membranes, the V-type H + ATPase is now also found in increasing examples of plasma membranes where the proton pump energizes transport across cell membranes and entire epithelia...

...John K. Zehmer; Jeffrey R. Hazel SUMMARY Rafts are cholesterol- and sphingolipid-enriched microdomains of the plasma membrane (PM) that organize many signal transduction pathways. Interactions between cholesterol and saturated lipids lead to patches of liquid-ordered membrane (rafts) phase...

...Dorothy M. Morré; Giorgio Lenaz; D. James Morré ABSTRACT This report summarizes new evidence for a plasma-membrane-associated hydroquinone oxidase designated as CNOX (constitutive plasma membrane NADH oxidase) that functions as a terminal oxidase for a plasma membrane oxidoreductase (PMOR) electron...

... , at least during the acclimation time course used in the present study. * e-mail: [email protected] 25 05 1999 09 08 1999 © 1999 by Company of Biologists 1999 Na + /K + -ATPase gill salinity adaptation plasma membrane eel Anguilla rostrata Na + pump...

...John C. Robertson; Jeffrey R. Hazel ABSTRACT Tissue homogenates from rainbow trout gill had three-to fivefold higher specific activity for 5´-nucleotidase (5´NT) and more than twofold greater alkaline phosphodiesterase (APD) activity than liver or kidney homogenates. In isolated plasma membranes...

... fraction was verified by immunoblots using an antiserum to the V-ATPase holoenzyme from Manduca sexta midgut. The antiserum localized the V-ATPase to the apical plasma membrane of the anterior silk gland cells, suggesting that the enzyme is functionally active in pumping protons out of the cell towards...

...Marcel J. C. Bijvelds; Zvonimir Kolar; Sjoerd E. Wendelaar Bonga; Gert Flik ABSTRACT To elucidate the mechanisms involved in Mg 2+ transport at the apical and basolateral poles of the renal tubular epithelium, apical and basolateral plasma membrane vesicle preparations were derived from kidney...

... Limited 1995 plasma membrane goblet cell gap junction 16 kDa proteolipid Lepidoptera larvae insect V-ATPase Heliothis virescens confocal microscopy The lepidopteran larval midgut epithelium is one cell thick and is composed of two main cell types, columnar and goblet cells...

...Alexandra Lepier; Masaaki Azuma; William R. Harvey; Helmut Wieczorek ABSTRACT The midgut of the tobacco hornworm secretes K + across the apical plasma membrane of its goblet cells. This secondary K + transport results from K + /H + antiport energized by the proton-motive force generated...

...Gert Flik; Pieter M. Verbost; Wim Atsma; Cedomil Lucu ABSTRACT A procedure was developed for the preparation of inside-out vesicles from plasma membranes isolated from the branchial epithelium of the green shore crab Carcinus maenas (L.). Procedures normally applied to fish branchial epithelium...

... tubules of M. sexta cross reacted with an immune serum directed to the purified plasma membrane V-ATPase from the midgut; the M. sexta midgut V-ATPase cross reacted with polyclonal antibodies to endomembrane V-ATPases from xenic origin. In immunocytochemical investigations of larvae of M. sexta and adults...

... by monoclonal antibodies to the A subunit of the Manduca V-ATPase or by hybridisation with a cDNA probe for a plant V-ATPase B subunit have been cloned and sequenced. There is a high degree of identity to the sequences of the respective subunits of other V-ATPases. The M. sexta plasma membrane V-ATPase...