... difference ( V te ) of isolated and perfused gills increased significantly when hyposmotic saline (699 mosmol l -1 ) was used instead of isosmotic solution (1045 mosmol l -1 ). A reduction of the concentration of Na + or Cl - at constant osmolarity did not produce any change in V te . Transepithelial short...

...Čedomil Lucu; Dietrich Siebers ABSTRACT Sodium and chloride fluxes, as well as transbranchial potentials (TBP) were studied in isolated perfused gill filaments of the crab Carcinus mediterraneus . Experiments were carried out in media that were either hyposmotic to the perfusion solution...