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Keywords: patch-clamp
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Journal Articles
J Exp Biol (2022) 225 (12): jeb244079.
Published: 14 June 2022
... or electrophysiological properties of putative respiratory neurons in slices without relevant synaptic inputs. Here, we detail a method to horizontally slice away the dorsal portion of the brainstem to expose fluorescently labeled motoneurons for patch-clamp recordings in American bullfrogs. This ‘semi-intact...
Includes: Supplementary data
Journal Articles
J Exp Biol (2007) 210 (3): 454–464.
Published: 1 February 2007
... species, action potentials in muscle fibres isolated from adult honeybee metathoracic tibia,are not graded but actual all-or-none responses. Action potentials are blocked by Cd 2+ and La 3+ but not by tetrodotoxin (TTX) in current-clamp mode of the patch-clamp technique, and as assessed under voltage...
Journal Articles
J Exp Biol (2001) 204 (2): 367–378.
Published: 15 January 2001
...Kevin R. O’connor; Klaus W. Beyenbach ABSTRACT Stellate cells of Aedes aegypti Malpighian tubules were investigated using patch-clamp methods to probe the route of transepithelial Cl − secretion. Two types of Cl − channel were identified in excised, inside-out apical membrane patches. The first Cl...
Journal Articles
Journal Articles
J Exp Biol (1998) 201 (5): 745–754.
Published: 1 March 1998
... of the patch-clamp technique ( Hamill et al . 1981 ) on cells maintained in short-term tissue culture at 19–21 °C. Abdominal ganglia with intact bag cell clusters from A. californica of known ages were removed from animals anesthetized for 30 min in a 1:1 mixture of isotonic MgCl 2 and sea water and were...
Journal Articles
J Exp Biol (1997) 200 (4): 723–733.
Published: 15 February 1997
...Donald E. R. Meyers; Ian M. Cooke ABSTRACT The whole-cell patch-clamp technique was used to examine Ca 2+ currents (I Ca ) in mature neurons cultured in defined medium and derived from the principal neurosecretory system of decapod crustaceans, the X-organ–sinus gland. After 1 day in culture, X...
Journal Articles
J Exp Biol (1996) 199 (5): 1115–1126.
Published: 1 May 1996
... a graded receptor potential into a train of action potentials. * Author for correspondence. 23 01 1996 ©The Company of Biologists Limited 1996 zebrafish Danio rerio Brachydanio rerio olfaction whole-cell currents patch-clamp Recently, our understanding...
Journal Articles
J Exp Biol (1996) 199 (3): 537–548.
Published: 1 March 1996
... currents are found to be much more stable than when measured using the conventional whole-cell (CWC) patch-clamp technique. Ca 2+ channel currents, which typically ‘wash out’ within a few minutes of the beginning of CWC recording, are stable for the duration of the seal (tens of minutes) when measured...
Journal Articles
J Exp Biol (1994) 196 (1): 263–281.
Published: 1 November 1994
...’, in which an increased electrostatic driving force can increase net charge flow. Studies are under way to exploit the power of patch-clamp techniques to develop new models for NET and SERT transport mechanisms and to gain insights into second-messenger control of transporter activity. These techniques also...
Journal Articles
J Exp Biol (1993) 177 (1): 201–221.
Published: 1 April 1993
...H. A. Pearson; G. Lees; D. Wray ABSTRACT Using the patch-clamp technique, Ca 2+ channel currents were recorded from neurones freshly isolated from the thoracic ganglia of the desert locust Schistocerca gregaria . In solutions containing 10mmol l −1 Ba 2+ we observed high-voltage-activated whole...
Journal Articles
J Exp Biol (1992) 172 (1): 289–309.
Published: 1 November 1992
... patch-clamp recordings of membrane capacitance and current fluctuations from MR cells demonstrate that a respiratory acid load and aldosterone produce rapid exocytotic insertion of DCCD-sensitive conductive membrane. A secondary role of the H + pump is to energize sodium absorption (JNa ) via principal...
Journal Articles
J Exp Biol (1992) 169 (1): 87–104.
Published: 1 August 1992
.... In this report, we identify a membrane ion channel that is sensitive to membrane tension; this cation-selective ion channel may constitute one mechanism for the transduction of mechanical stimuli. In contrast to several other types of ion channels exhibiting spontaneous activity in our patch-clamp study...
Journal Articles
J Exp Biol (1992) 166 (1): 1–17.
Published: 1 May 1992
...Gabrielle A. Nevitt; William J. Moody ABSTRACT Electrical properties of ciliated olfactory receptor cells isolated from coho salmon (Oncorhynchus kisutch) were studied using the whole-cell mode of the patch-clamp recording technique. Voltage-dependent currents could be separated into two inward...
Journal Articles
Journal Articles
J Exp Biol (1992) 162 (1): 231–249.
Published: 1 January 1992
...Mary T. Lucero; Frank T. Horrigan; Wm F. Gilly ABSTRACT Electrical properties of isolated olfactory receptor cells were studied using voltage- and current-clamp techniques based on whole-cell patch-clamp methods. Squid olfactory receptor cells contain voltage-gated Na + and K + channels...
Journal Articles
J Exp Biol (1991) 159 (1): 45–64.
Published: 1 September 1991
...CHRISTOPHER A. LORETZ; CHARLES R. FOURTNER Using patch-clamp techniques, a Ca 2+ -dependent, voltage-gated K + channel [K(Ca) channel] was isolated from the basolateral membrane of NaCl-absorbing intestinal epithelial cells of the goby Gillichthys mirabilis . This K(Ca) channel had a high...
Journal Articles
J Exp Biol (1991) 156 (1): 607–610.
Published: 1 March 1991
...C. A. Bishop; M. E. Krouse; J. J. Wine ABSTRACT Using the single-channel patch-clamp technique we have recently shown that the peptide cotransmitter proctolin can potentiate the activity of a large (38 pS in 137 mmol l −1 Ba 2+ ; 26 pS in 137 mmol l −1 Ca 2+ ), depolarization-inducible Ca 2...
Journal Articles
J Exp Biol (1991) 156 (1): 619–623.
Published: 1 March 1991
... photoreceptors ( Alkon, 1984 , 1987 ; Alkon et al . 1985 ). We have used the isolated eye preparation to characterize two types of potassium channels in photoreceptor plasma membranes. 31 10 1990 © 1991 by Company of Biologists 1991 Hermissenda crassicornis photoreceptor patch-clamp K...
Journal Articles
Journal Articles
J Exp Biol (1990) 154 (1): 45–65.
Published: 1 November 1990
...B. A. Miller; P. N. R. Usherwood ABSTRACT K + channel activity in cultured locust myofibres was investigated using gigaohm patch-clamp techniques. After 2 months in vitro the myofibres had a mean resting potential of -39±7mV (±S.D., N =42). Five types of K + channel were identified at this time...