... or electrophysiological properties of putative respiratory neurons in slices without relevant synaptic inputs. Here, we detail a method to horizontally slice away the dorsal portion of the brainstem to expose fluorescently labeled motoneurons for patch-clamp recordings in American bullfrogs. This ‘semi-intact...

... species, action potentials in muscle fibres isolated from adult honeybee metathoracic tibia,are not graded but actual all-or-none responses. Action potentials are blocked by Cd 2+ and La 3+ but not by tetrodotoxin (TTX) in current-clamp mode of the patch-clamp technique, and as assessed under voltage...

...Kevin R. O’connor; Klaus W. Beyenbach ABSTRACT Stellate cells of Aedes aegypti Malpighian tubules were investigated using patch-clamp methods to probe the route of transepithelial Cl − secretion. Two types of Cl − channel were identified in excised, inside-out apical membrane patches. The first Cl...

...-barrelled stimulating pipette as explained in Fig. 7 . Data were sampled at 500 Hz and displayed using a compression factor of 10. * Author for correspondence (e-mail: [email protected] ) 18 08 1999 22 12 1999 © 2000 by Company of Biologists 2000 patch-clamp...

... of the patch-clamp technique ( Hamill et al . 1981 ) on cells maintained in short-term tissue culture at 19–21 °C. Abdominal ganglia with intact bag cell clusters from A. californica of known ages were removed from animals anesthetized for 30 min in a 1:1 mixture of isotonic MgCl 2 and sea water and were...

...Donald E. R. Meyers; Ian M. Cooke ABSTRACT The whole-cell patch-clamp technique was used to examine Ca 2+ currents (I Ca ) in mature neurons cultured in defined medium and derived from the principal neurosecretory system of decapod crustaceans, the X-organ–sinus gland. After 1 day in culture, X...

....91.24.11646 Barry , P. H. and Lynch , J. W. ( 1991 ). Liquid junction potentials and small cell effects in patch-clamp analysis . J. Membr. Biol. 121 , 101 – 117 . 10.1007/BF01870526 Bloom , H. D. and Perlmutter , A. ( 1977 ). A sexual aggregating pheromone system...

... currents are found to be much more stable than when measured using the conventional whole-cell (CWC) patch-clamp technique. Ca 2+ channel currents, which typically ‘wash out’ within a few minutes of the beginning of CWC recording, are stable for the duration of the seal (tens of minutes) when measured...

... on single cells subjected to whole-cell patch-clamp techniques. NET currents are elicited by application of guanethidine, a more stable substrate for NET than NE (NE currents can also be detected, although they are generally of smaller magnitude). SERT currents are observed after application of 5-HT. A slow...

...H. A. Pearson; G. Lees; D. Wray ABSTRACT Using the patch-clamp technique, Ca 2+ channel currents were recorded from neurones freshly isolated from the thoracic ganglia of the desert locust Schistocerca gregaria . In solutions containing 10mmol l −1 Ba 2+ we observed high-voltage-activated whole...

... patch-clamp recordings of membrane capacitance and current fluctuations from MR cells demonstrate that a respiratory acid load and aldosterone produce rapid exocytotic insertion of DCCD-sensitive conductive membrane. A secondary role of the H + pump is to energize sodium absorption (JNa ) via principal...

... stretch-activated channel fish intestinal epithelium patch-clamp Gillichthys mirabilis It has been well documented that the intestine is an important osmoregulatory organ in teleosts, especially in those euryhaline teleosts encountering variations in the external osmotic environment. Ion flux...

...Gabrielle A. Nevitt; William J. Moody ABSTRACT Electrical properties of ciliated olfactory receptor cells isolated from coho salmon (Oncorhynchus kisutch) were studied using the whole-cell mode of the patch-clamp recording technique. Voltage-dependent currents could be separated into two inward...

... of Biologists 1992 sodium channels patch-clamp cultured neurones insect deltamethrin Periplaneta americana Agents that modify the properties of membrane ionic channels are useful tools for deciphering channel structure and function. Several molecules are known to activate the sodium...

...Mary T. Lucero; Frank T. Horrigan; Wm F. Gilly ABSTRACT Electrical properties of isolated olfactory receptor cells were studied using voltage- and current-clamp techniques based on whole-cell patch-clamp methods. Squid olfactory receptor cells contain voltage-gated Na + and K + channels...

...CHRISTOPHER A. LORETZ; CHARLES R. FOURTNER Using patch-clamp techniques, a Ca 2+ -dependent, voltage-gated K + channel [K(Ca) channel] was isolated from the basolateral membrane of NaCl-absorbing intestinal epithelial cells of the goby Gillichthys mirabilis . This K(Ca) channel had a high...

...C. A. Bishop; M. E. Krouse; J. J. Wine ABSTRACT Using the single-channel patch-clamp technique we have recently shown that the peptide cotransmitter proctolin can potentiate the activity of a large (38 pS in 137 mmol l −1 Ba 2+ ; 26 pS in 137 mmol l −1 Ca 2+ ), depolarization-inducible Ca 2...

... photoreceptors ( Alkon, 1984 , 1987 ; Alkon et al . 1985 ). We have used the isolated eye preparation to characterize two types of potassium channels in photoreceptor plasma membranes. 31 10 1990 © 1991 by Company of Biologists 1991 Hermissenda crassicornis photoreceptor patch-clamp K...

... of the open and closed durations.) 14 6 1990 © 1990 by Company of Biologists 1990 acetylcholine channel patch-clamp Ascaris suum muscle Ascaris suum is a parasitic nematode which lives in the intestine of the pig and is a target species for a variety of anthelmintic...

...B. A. Miller; P. N. R. Usherwood ABSTRACT K + channel activity in cultured locust myofibres was investigated using gigaohm patch-clamp techniques. After 2 months in vitro the myofibres had a mean resting potential of -39±7mV (±S.D., N =42). Five types of K + channel were identified at this time...