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1-20 of 29
Keywords: patch-clamp
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Journal Articles
Journal:
Journal of Experimental Biology
J Exp Biol (2022) 225 (12): jeb244079.
Published: 14 June 2022
... or electrophysiological properties of putative respiratory neurons in slices without relevant synaptic inputs. Here, we detail a method to horizontally slice away the dorsal portion of the brainstem to expose fluorescently labeled motoneurons for patch-clamp recordings in American bullfrogs. This ‘semi-intact...
Includes: Supplementary data
Journal Articles
Journal:
Journal of Experimental Biology
J Exp Biol (2007) 210 (3): 454–464.
Published: 1 February 2007
... species, action potentials in muscle fibres isolated from adult honeybee metathoracic tibia,are not graded but actual all-or-none responses. Action potentials are blocked by Cd 2+ and La 3+ but not by tetrodotoxin (TTX) in current-clamp mode of the patch-clamp technique, and as assessed under voltage...
Journal Articles
Chloride Channels in Apical Membrane Patches of Stellate Cells of Malpighian Tubules of Aedes Aegypti
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Journal:
Journal of Experimental Biology
J Exp Biol (2001) 204 (2): 367–378.
Published: 15 January 2001
...Kevin R. O’connor; Klaus W. Beyenbach ABSTRACT Stellate cells of Aedes aegypti Malpighian tubules were investigated using patch-clamp methods to probe the route of transepithelial Cl − secretion. Two types of Cl − channel were identified in excised, inside-out apical membrane patches. The first Cl...
Journal Articles
The Contribution of a Ca 2+ -Activated Cl − Conductance to Amino-Acid-induced Inward Current Responses of Ciliated Olfactory Neurons of the Rainbow Trout
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Journal:
Journal of Experimental Biology
J Exp Biol (2000) 203 (2): 253–262.
Published: 15 January 2000
...-barrelled stimulating pipette as explained in Fig. 7 . Data were sampled at 500 Hz and displayed using a compression factor of 10. * Author for correspondence (e-mail: [email protected] ) 18 08 1999 22 12 1999 © 2000 by Company of Biologists 2000 patch-clamp...
Journal Articles
Characterization of Na + and Ca 2+ Currents in Bag Cells of Sexually Immature Aplysia Californica
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Journal:
Journal of Experimental Biology
J Exp Biol (1998) 201 (5): 745–754.
Published: 1 March 1998
... of the patch-clamp technique ( Hamill et al . 1981 ) on cells maintained in short-term tissue culture at 19–21 °C. Abdominal ganglia with intact bag cell clusters from A. californica of known ages were removed from animals anesthetized for 30 min in a 1:1 mixture of isotonic MgCl 2 and sea water and were...
Journal Articles
Comparison of Ca 2+ Currents of Peptidergic Neurons Developing Differing Morphology with Time in Culture
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Journal:
Journal of Experimental Biology
J Exp Biol (1997) 200 (4): 723–733.
Published: 15 February 1997
...Donald E. R. Meyers; Ian M. Cooke ABSTRACT The whole-cell patch-clamp technique was used to examine Ca 2+ currents (I Ca ) in mature neurons cultured in defined medium and derived from the principal neurosecretory system of decapod crustaceans, the X-organ–sinus gland. After 1 day in culture, X...
Journal Articles
Voltage- and Ca 2+ -Gated Currents in Zebrafish Olfactory Receptor Neurons
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Journal:
Journal of Experimental Biology
J Exp Biol (1996) 199 (5): 1115–1126.
Published: 1 May 1996
....91.24.11646 Barry , P. H. and Lynch , J. W. ( 1991 ). Liquid junction potentials and small cell effects in patch-clamp analysis . J. Membr. Biol. 121 , 101 – 117 . 10.1007/BF01870526 Bloom , H. D. and Perlmutter , A. ( 1977 ). A sexual aggregating pheromone system...
Journal Articles
Modulation of Membrane Currents by Cyclic Amp in Cleavage-Arrested Drosophila Neurons
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Journal:
Journal of Experimental Biology
J Exp Biol (1996) 199 (3): 537–548.
Published: 1 March 1996
... currents are found to be much more stable than when measured using the conventional whole-cell (CWC) patch-clamp technique. Ca 2+ channel currents, which typically ‘wash out’ within a few minutes of the beginning of CWC recording, are stable for the duration of the seal (tens of minutes) when measured...
Journal Articles
Molecular Physiology of Norepinephrine and Serotonin Transporters
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Journal:
Journal of Experimental Biology
J Exp Biol (1994) 196 (1): 263–281.
Published: 1 November 1994
... on single cells subjected to whole-cell patch-clamp techniques. NET currents are elicited by application of guanethidine, a more stable substrate for NET than NE (NE currents can also be detected, although they are generally of smaller magnitude). SERT currents are observed after application of 5-HT. A slow...
Journal Articles
Calcium Channel Currents In Neurones From Locust ( Schistocerca Gregaria ) Thoracic Ganglia
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Journal:
Journal of Experimental Biology
J Exp Biol (1993) 177 (1): 201–221.
Published: 1 April 1993
...H. A. Pearson; G. Lees; D. Wray ABSTRACT Using the patch-clamp technique, Ca 2+ channel currents were recorded from neurones freshly isolated from the thoracic ganglia of the desert locust Schistocerca gregaria . In solutions containing 10mmol l −1 Ba 2+ we observed high-voltage-activated whole...
Journal Articles
Energization of sodium absorption by the H + -ATPase pump in mitochondria-rich cells of frog skin
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Journal:
Journal of Experimental Biology
J Exp Biol (1992) 172 (1): 289–309.
Published: 1 November 1992
... patch-clamp recordings of membrane capacitance and current fluctuations from MR cells demonstrate that a respiratory acid load and aldosterone produce rapid exocytotic insertion of DCCD-sensitive conductive membrane. A secondary role of the H + pump is to energize sodium absorption (JNa ) via principal...
Journal Articles
Activation by Membrane Stretch and Depolarization of an Epithelial Monovalent Cation Channel From Teleost Intestine
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Journal:
Journal of Experimental Biology
J Exp Biol (1992) 169 (1): 87–104.
Published: 1 August 1992
... stretch-activated channel fish intestinal epithelium patch-clamp Gillichthys mirabilis It has been well documented that the intestine is an important osmoregulatory organ in teleosts, especially in those euryhaline teleosts encountering variations in the external osmotic environment. Ion flux...
Journal Articles
An Electrophysiological Characterization of Ciliated Olfactory Receptor Cells of the Coho Salmon Oncorhynchus Kisutch
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Journal:
Journal of Experimental Biology
J Exp Biol (1992) 166 (1): 1–17.
Published: 1 May 1992
...Gabrielle A. Nevitt; William J. Moody ABSTRACT Electrical properties of ciliated olfactory receptor cells isolated from coho salmon (Oncorhynchus kisutch) were studied using the whole-cell mode of the patch-clamp recording technique. Voltage-dependent currents could be separated into two inward...
Journal Articles
Patch-Clamp Analysis of the Effects of the Insecticide Deltamethrin on Insect Neurones
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Journal:
Journal of Experimental Biology
J Exp Biol (1992) 163 (1): 65–84.
Published: 1 February 1992
... of Biologists 1992 sodium channels patch-clamp cultured neurones insect deltamethrin Periplaneta americana Agents that modify the properties of membrane ionic channels are useful tools for deciphering channel structure and function. Several molecules are known to activate the sodium...
Journal Articles
Electrical Responses to Chemical Stimulation of Squid Olfactory Receptor Cells
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Journal:
Journal of Experimental Biology
J Exp Biol (1992) 162 (1): 231–249.
Published: 1 January 1992
...Mary T. Lucero; Frank T. Horrigan; Wm F. Gilly ABSTRACT Electrical properties of isolated olfactory receptor cells were studied using voltage- and current-clamp techniques based on whole-cell patch-clamp methods. Squid olfactory receptor cells contain voltage-gated Na + and K + channels...
Journal Articles
Identification of a Basolateral Membrane Potassium Channel from Teleost Intestinal Epithelial Cells
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Journal:
Journal of Experimental Biology
J Exp Biol (1991) 159 (1): 45–64.
Published: 1 September 1991
...CHRISTOPHER A. LORETZ; CHARLES R. FOURTNER Using patch-clamp techniques, a Ca 2+ -dependent, voltage-gated K + channel [K(Ca) channel] was isolated from the basolateral membrane of NaCl-absorbing intestinal epithelial cells of the goby Gillichthys mirabilis . This K(Ca) channel had a high...
Journal Articles
Peptide Potentiation of Calcium Channel Activity can be Seasonally Variable
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Journal:
Journal of Experimental Biology
J Exp Biol (1991) 156 (1): 607–610.
Published: 1 March 1991
...C. A. Bishop; M. E. Krouse; J. J. Wine ABSTRACT Using the single-channel patch-clamp technique we have recently shown that the peptide cotransmitter proctolin can potentiate the activity of a large (38 pS in 137 mmol l −1 Ba 2+ ; 26 pS in 137 mmol l −1 Ca 2+ ), depolarization-inducible Ca 2...
Journal Articles
Gigaohm Single-Channel Recording From Isolated Hermissenda Crassicornis Type B Photoreceptors
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Journal:
Journal of Experimental Biology
J Exp Biol (1991) 156 (1): 619–623.
Published: 1 March 1991
... photoreceptors ( Alkon, 1984 , 1987 ; Alkon et al . 1985 ). We have used the isolated eye preparation to characterize two types of potassium channels in photoreceptor plasma membranes. 31 10 1990 © 1991 by Company of Biologists 1991 Hermissenda crassicornis photoreceptor patch-clamp K...
Journal Articles
A Patch-Clamp Study of Acetylcholine-Activated Ion Channels in Ascaris Suum Muscle
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Journal:
Journal of Experimental Biology
J Exp Biol (1990) 154 (1): 201–221.
Published: 1 November 1990
... of the open and closed durations.) 14 6 1990 © 1990 by Company of Biologists 1990 acetylcholine channel patch-clamp Ascaris suum muscle Ascaris suum is a parasitic nematode which lives in the intestine of the pig and is a target species for a variety of anthelmintic...
Journal Articles
Characteristics of Five Types of K + Channel in Cultured Locust Muscle
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Journal:
Journal of Experimental Biology
J Exp Biol (1990) 154 (1): 45–65.
Published: 1 November 1990
...B. A. Miller; P. N. R. Usherwood ABSTRACT K + channel activity in cultured locust myofibres was investigated using gigaohm patch-clamp techniques. After 2 months in vitro the myofibres had a mean resting potential of -39±7mV (±S.D., N =42). Five types of K + channel were identified at this time...
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