... processes and energy consumption due to homeostatic regulation of the midgut. While it is obvious that the digestive system is the major organ in marine larvae that controls the animal's energy budget, it remained unknown which digestive enzymes are particularly involved in digestive function. The present...

.... Using a specific antibody raised against DRIP in the silkworm Bombyx mori (AQP-Bom1), Anocu AQP1 was localised to the apical plasma membrane of rectal epithelial cells, and lacking in the midgut and gastric caecal epithelia. Based on the BeetleBase prediction, there are three putative AQPs (Trica-3a, 3b...

...) in position 3 and methionine (M) in position 8. Reverse transcriptase (RT)-PCR shows that Rhopr-MS is expressed in the CNS and posterior midgut in R. prolixus and immunohistochemistry suggests that an RFamide-like peptide is present in endocrine-like cells in the midgut. Physiological assays using Rhopr-MS...

... tissues is critical for oogenesis and other physiological processes. We have identified a proton amino acid transporter (PAT) in Aedes aegypti (AaePAT1, AAEL007191) which facilitates this transport and is expressed in epithelial cell membranes of larval caecae and the adult midgut. AaePAT1 encodes a 475...

...Paul J. Linser; Kristin E. Smith; Terri J. Seron; Marco Neira Oviedo SUMMARY Mosquito larvae use a digestive strategy that is relatively rare in nature. The anterior half of the larval mosquito midgut has a luminal pH that ranges between 10.5 and 11.5. Most other organisms, both large and small...

... and multidrug resistance-associated proteins in the excretion of toxins. e-mail: odonnell@mcmaster.ca 18 9 2008 2009 insect Malpighian tubule midgut ion-selective microelectrodes excretion dietary toxins haematophagy When a blood-feeding insect such as Rhodnius ingests...

...Gunnar Broehan; Lars Zimoch; Anton Wessels; Beyhan Ertas; Hans Merzendorfer SUMMARY The chitin portion of the peritrophic matrix in the midgut of the tobacco hornworm, Manduca sexta , is produced by chitin synthase 2 (CHS2), a transmembrane family II glycosyltransferase, located at the apical tips...

... membrane of Malpighian tubule, midgut and the ion-transporting sector of gastric caeca. Midgut expression of NHE3 shows a different pattern of enrichment between larval and adult stages, implicating it in the maintenance of regional pH in the midgut during the life cycle. In all tissues examined, NHE3...

... in vivo models are required. The present study focuses on the dynamics of a new and simple model of intestinal epithelial regeneration found in the midgut of the migratory locust, Locusta migratoria (Linnaeus 1758). The locust midgut consists of three cell types: columnar cells, endocrine cells...

... allatostatin allatotropin anterior stomach head peptide larva midgut mosquito neuropeptide F peristalsis proctolin transepithelial voltage In addition to its conventional roles in food digestion and nutrient absorption, the stomach of mosquito larvae is of critical importance for ionic...

...) in the epithelial cells of the midgut (with special reference to the midgut caeca) in the African migratory locust, Locusta migratoria . Densitometric analysis of PCNA-immunostained cells was used as an indirect measure of the levels of expression of PCNA, and a PCNA cellular index(PCNA-I) was obtained...

... granulocytes of the polychaete annelid Nereis diversicolor by a monoclonal antibody against a cadmium-binding protein (MPII). Biol. Cell. 60 , 259 -261. Punin, M. Y. and Lukyanova, E. L. ( 1984 ). Midgut epithelium organisation of the errant polychaete Nereis virens . 1 Absorptive and secretory...

...Maria del Pilar Corena; Theresa J. Seron; Herm K. Lehman; Judith D. Ochrietor; Andrea Kohn; Chingkuang Tu; Paul J. Linser SUMMARY The larval mosquito midgut exhibits one of the highest pH values known in a biological system. While the pH inside the posterior midgut and gastric caeca ranges between...

...Helmut Wieczorek; Gerhard Grüber; William R. Harvey; Markus Huss; Hans Merzendorfer; Wolfgang Zeiske ABSTRACT H + V-ATPases (V-ATPases) are found in two principal locations, in endomembranes and in plasma membranes. The plasma membrane V-ATPase from the midgut of larval Manduca sexta is the sole...

...Zhengpeng Zhuang; Paul J. Linser; William R. Harvey ABSTRACT The pH profile, gross structure, ultrastructure and immunolabeling of the mosquito ( Aedes aegypti ) larval midgut are described as a first step in analyzing the role of plasma membrane H+ V-ATPase in the alkalization of the gut, nutrient...

...Helge Zieler; Joseph P. Nawrocki; Mohammed Shahabuddin ABSTRACT During the course of its development in the mosquito and transmission to a new vertebrate host, the malaria parasite must interact with the mosquito midgut and invade the gut epithelium. To investigate how the parasite recognizes...

...Thomas M. Clark; Alan Koch; David F. Moffett ABSTRACT The ‘stomach’ region of the larval mosquito midgut is divided into histologically distinct anterior and posterior regions. Anterior stomach perfused symmetrically with saline in vitro had an initial transepithelial potential (TEP) of −66 mV...

...Enrique Zudaire; Steve J. Simpson; Luis M. Montuenga ABSTRACT We have studied the influence of variations in dietary protein and digestible carbohydrate content, of insect age and of time during the feeding cycle on the endocrine cells of the ampullar region of the midgut in the African migratory...

...Kyeong-Yeoll Lee; Frank M. Horodyski; M. E. Chamberlin ABSTRACT Short-circuit current ( I sc ) across the posterior midgut of day 2 fifth-instar tobacco hornworms ( Manduca sexta ) is inhibited by Manduca allatotropin (Mas-AT) and two Manduca FLRFamides (F7G and F7D). Another FLRFamide, F10...

...M. E. Chamberlin ABSTRACT Mitochondria isolated from the posterior midgut of the tobacco hornworm ( Manduca sexta ) contain arginine kinase. The distribution of mitochondrial and cytoplasmic marker enzymes indicates that the presence of mitochondrial arginine kinase is not due to cytoplasmic...