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Keywords: microarray
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Journal Articles
J Exp Biol (2015) 218 (12): 1915–1924.
Published: 01 June 2015
... expression Microarray Non-model species RNAseq Stenotherm Thus, it is challenging to compare transcriptional responses among studies, particularly on a gene-by-gene basis. Therefore, we focus on summarizing and synthesizing key findings from studies of stenothermal versus extremely eurythermal...
Journal Articles
J Exp Biol (2015) 218 (12): 1925–1935.
Published: 01 June 2015
... of transcriptomics to identify genes that underlie environmental adaptation is explored in the context of recent systems-level experiments that provide new insights into the relationship between gene expression and fitness. Environment Microarray Physiology RNA-seq Stress Temperature Transcriptomics...
Journal Articles
J Exp Biol (2012) 215 (8): 1293–1305.
Published: 15 April 2012
... ). A microarray-based transcriptomic time-course of hyper- and hypo-osmotic stress signaling events in the euryhaline fish Gillichthys mirabilis: osmosensors to effectors . J. Exp. Biol. 211 , 3636 – 3649 . Faggian J. , Fosang A. J. , Zieba M. , Wallace M. J. , Hooper S. B...
Includes: Supplementary data
Journal Articles
J Exp Biol (2011) 214 (23): 3948–3960.
Published: 01 December 2011
... mechanisms regulating dormancy in animals remain poorly understood. In insects, dormancy (diapause) is a developmentally dynamic state, and the mechanisms that control diapause transitions affect seasonal timing. Here we used microarrays to examine patterns of gene expression during dormancy termination...
Includes: Supplementary data
Journal Articles
J Exp Biol (2011) 214 (23): 4021–4029.
Published: 01 December 2011
..., a single 10 h exposure, or five 2 h exposures on consecutive days, and extracted RNA after 6 h of recovery. Global gene expression was quantified using an oligonucleotide microarray and validated with real-time PCR using different biological replicates. We identified 76 genes upregulated in response...
Includes: Supplementary data
Journal Articles
J Exp Biol (2011) 214 (18): 3005–3012.
Published: 15 September 2011
... met by glucose stored as glycogen in tissues such as liver ( Richards, 2011 ). The changes in enzyme activity required for metabolic reorganization during hypoxia are often regulated by the differential expression of mRNA ( Semenza et al., 1996 ). Here we apply microarray-based gene expression...
Includes: Supplementary data
Journal Articles
J Exp Biol (2011) 214 (6): 979–987.
Published: 15 March 2011
... at both the morning and afternoon training times to result in one set of bees that was undergoing stereotypical food anticipatory behavior and another that was inactive for each time of day. Between the two groups with the different spatiotemporal memories, microarray analysis revealed that 1329 genes...
Includes: Supplementary data
Journal Articles
J Exp Biol (2010) 213 (20): 3548–3558.
Published: 15 October 2010
... traits may underlie the capacity of M. galloprovincialis to out-compete M. trossulus in warm habitats. To elucidate possible differences in stress-induced gene expression between these congeners, we developed an oligonucleotide microarray with 8874 probes representing 4488 different genes that recognized...
Includes: Supplementary data
Journal Articles
J Exp Biol (2009) 212 (16): 2579–2594.
Published: 15 August 2009
... species possess the physiological capacity to compensate for the potentially adverse effects of ocean acidification. We carried out a microarray-based transcriptomic analysis of the physiological response of larvae of a calcifying marine invertebrate, the purple sea urchin, Strongylocentrotus purpuratus...
Journal Articles
J Exp Biol (2008) 211 (22): 3636–3649.
Published: 15 November 2008
... of the osmosensing and signal transduction genes that may govern their activities. To better resolve this issue, a cDNA microarray consisting of 9207 cDNA clones was used to monitor gene expression changes in the gill of the euryhaline fish Gillichthys mirabilis exposed to hyper- and hypo-osmotic stress. We...
Includes: Supplementary data
Journal Articles
J Exp Biol (2008) 211 (18): 3041–3056.
Published: 15 September 2008
..., Canada, H3C 3J7 15 7 2008 © The Company of Biologists Limited 2008 2008 cichlid microarray social behavior behavior plasticity Brain and behavior are sculpted by a dynamic interplay between genotype and environment. Changes in social status mediate changes in a wide range...
Includes: Supplementary data
Journal Articles
J Exp Biol (2007) 210 (9): 1507–1517.
Published: 01 May 2007
... to understand the link between genotype,environment and phenotype. While genomic technologies such as DNA microarrays,proteomics and metabolomics have given us new tools and new sources of data to address these problems, a number of crucial elements remain to be addressed before we can begin to close the loop...
Includes: Supplementary data
Journal Articles
J Exp Biol (2007) 210 (9): 1584–1592.
Published: 01 May 2007
..., and each method has specific advantages and disadvantages. Construction of custom cDNA microarrays remains the most popular route to implement expression screens in the non-model organisms favored by comparative physiologists, and we highlight some factors that should be considered when embarking along...
Includes: Supplementary data
Journal Articles
J Exp Biol (2007) 210 (9): 1613–1621.
Published: 01 May 2007
... there is a complexity in how gene expression is related to physiological processes. * Author for correspondence (e-mail: dcrawford@rsmas.miami.edu ) 6 3 2007 © The Company of Biologists Limited 2007 2007 functional genomics cardiac metabolism microarray evolutionary selection Fundulus...
Includes: Supplementary data
Journal Articles
J Exp Biol (2006) 209 (12): 2328–2336.
Published: 15 June 2006
... manifestations. Construction of a cDNA microarray led to a survey of transcript expression levels in seven tissues of carp, as a function of time,and three different extents of cooling. The resulting data delineated a common stress response found in all tissues that comprises genes involved in cellular...
Journal Articles
J Exp Biol (2006) 209 (12): 2239–2248.
Published: 15 June 2006
... exercise endurance hypoxia gene transcriptome morphometry microarray PCR Skeletal muscle's malleability, which enables remodeling of the muscle's structural makeup according to alterations in demand, is a particularly striking phenomenon in the animal kingdom. This plasticity is reflected...