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Keywords: microarray
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Journal Articles
J Exp Biol (2015) 218 (12): 1915–1924.
Published: 1 June 2015
.... Since then, technological advances from cDNA and oligonucleotide microarrays to RNA sequencing have made it relatively cheap and easy to examine transcriptome-wide changes in virtually any non-model species ( Gracey and Cossins, 2003 ; Ekblom and Galindo, 2011 ). RNA sequencing is currently replacing...
Journal Articles
J Exp Biol (2015) 218 (12): 1925–1935.
Published: 1 June 2015
... microarray or RNA sequencing dataset, sequenced genome or expressed sequence tag collection, the number of these taxonomically restricted genes (also referred to as ‘orphan’ or ‘unknown’ genes) proliferates ( Khalturin et al., 2009 ). Clearly, this is a major obstacle to elucidating the genetic basis for any...
Journal Articles
Journal Articles
Journal Articles
J Exp Biol (2011) 214 (23): 4021–4029.
Published: 1 December 2011
..., a single 10 h exposure, or five 2 h exposures on consecutive days, and extracted RNA after 6 h of recovery. Global gene expression was quantified using an oligonucleotide microarray and validated with real-time PCR using different biological replicates. We identified 76 genes upregulated in response...
Includes: Supplementary data
Journal Articles
J Exp Biol (2011) 214 (18): 3005–3012.
Published: 15 September 2011
... to A.Y.G. The authors thank Dr Gary Schoolnik and Dr George Somero of Stanford University for use of their laboratory for the printing and hybridization of the microarray slides. We are grateful to Dr Rudolf Zechner, University of Graz, Austria, for the anti-mouse ATGL antibody. REFERENCES...
Includes: Supplementary data
Journal Articles
J Exp Biol (2011) 214 (6): 979–987.
Published: 15 March 2011
... at both the morning and afternoon training times to result in one set of bees that was undergoing stereotypical food anticipatory behavior and another that was inactive for each time of day. Between the two groups with the different spatiotemporal memories, microarray analysis revealed that 1329 genes...
Includes: Supplementary data
Journal Articles
J Exp Biol (2010) 213 (20): 3548–3558.
Published: 15 October 2010
... traits may underlie the capacity of M. galloprovincialis to out-compete M. trossulus in warm habitats. To elucidate possible differences in stress-induced gene expression between these congeners, we developed an oligonucleotide microarray with 8874 probes representing 4488 different genes that recognized...
Includes: Supplementary data
Journal Articles
J Exp Biol (2009) 212 (16): 2579–2594.
Published: 15 August 2009
... species possess the physiological capacity to compensate for the potentially adverse effects of ocean acidification. We carried out a microarray-based transcriptomic analysis of the physiological response of larvae of a calcifying marine invertebrate, the purple sea urchin, Strongylocentrotus purpuratus...
Journal Articles
Journal Articles
J Exp Biol (2008) 211 (18): 3041–3056.
Published: 15 September 2008
..., Canada, H3C 3J7 15 7 2008 © The Company of Biologists Limited 2008 2008 cichlid microarray social behavior behavior plasticity Brain and behavior are sculpted by a dynamic interplay between genotype and environment. Changes in social status mediate changes in a wide range...
Includes: Supplementary data
Journal Articles
J Exp Biol (2007) 210 (9): 1507–1517.
Published: 1 May 2007
... to understand the link between genotype,environment and phenotype. While genomic technologies such as DNA microarrays,proteomics and metabolomics have given us new tools and new sources of data to address these problems, a number of crucial elements remain to be addressed before we can begin to close the loop...
Includes: Supplementary data
Journal Articles
J Exp Biol (2007) 210 (9): 1584–1592.
Published: 1 May 2007
..., and each method has specific advantages and disadvantages. Construction of custom cDNA microarrays remains the most popular route to implement expression screens in the non-model organisms favored by comparative physiologists, and we highlight some factors that should be considered when embarking along...
Includes: Supplementary data
Journal Articles
J Exp Biol (2007) 210 (9): 1613–1621.
Published: 1 May 2007
... correlations for expression! Although these opposite patterns of gene expression present a biochemical conundrum, an obvious molecular reason is that many of these enzymes share common transcription factors. Our microarray analyses of cardiac physiology demonstrated that >80% of the variation...
Includes: Supplementary data
Journal Articles
J Exp Biol (2006) 209 (12): 2239–2248.
Published: 15 June 2006
... ) or hypoxia (10.5% O 2 , N =6/group). M. solei were harvested from the four experimental groups and analyzed with custom-designed microarrays for expression of 222 muscle-relevant transcripts( Fluck et al., 2005a ; Dapp et al., 2004 ). The hypoxia-to-normoxia signal ratio of 142 detected transcripts...
Journal Articles
J Exp Biol (2006) 209 (12): 2328–2336.
Published: 15 June 2006
... manifestations. Construction of a cDNA microarray led to a survey of transcript expression levels in seven tissues of carp, as a function of time,and three different extents of cooling. The resulting data delineated a common stress response found in all tissues that comprises genes involved in cellular...