... by The Company of Biologists Ltd 2024 Summary: The scientific method, with its strict emphasis on hypothesis testing, can sometimes feel limiting when asking open questions (‘how? why?’). Can ‘big data’ offer a solution? FAIR principles Transcriptomics Proteomics Metabolomics Scientific method...

.... By using metabolomics techniques, we investigated changes in the metabolic network responses in Thoroughbred horses to high-intensity interval exercise performed with two distinct (15 min or 2 min) rest intervals. The peak plasma lactate level was higher during high-intensity exercise with a 2 min rest...

.... This response is called ‘heat hardening’ as it leads to the generation of phenotypes with increased heat tolerance. The aim of this study was to investigate the impact of heat hardening on the metabolomic profile of Mytilus galloprovincialis in order to identify the associated adjustments of biochemical...

... the effects of chronic warming by profiling the metabolomes of Vermont pupae via untargeted LC-MS metabolomics. Warming caused significant changes in abundance of hundreds of metabolites across the metabolome. Notably, there were warming-induced shifts in key biochemical pathways, such as pyruvate metabolism...

...Alex S. Torson; Yun-wei Dong; Brent J. Sinclair ‘Omics’ methods, such as transcriptomics, proteomics, lipidomics or metabolomics, yield simultaneous measurements of many related molecules in a sample. These approaches have opened new opportunities to generate and test hypotheses about...

...Eric J. Koch; Silvia Moriano-Gutierrez; Edward G. Ruby; Margaret McFall-Ngai; Manuel Liebeke ABSTRACT Associations between animals and microbes affect not only the immediate tissues where they occur, but also the entire host. Metabolomics, the study of small biomolecules generated during metabolic...

... for macronutrients. By choosing any combination of foods, dogs and cats could individually set their macronutrient intake. Plasma metabolomic profiles were determined at baseline and after animals had consumed their food intake of choice for 28 days. Based on food intake calculations over 28 days, dogs on average...

... and, in addition, we discuss how these comparative differences may represent an evolutionary pathway toward more extreme cold tolerance of insects. Fig. 6. Model based on metabolomics data, to identify metabolite profiles that are predictive of Drosophila cold tolerance. See Materials and methods...

...% to 12.6% in the larvae fed standard diet (agar, sugar, yeast, cornmeal). Next, we fed the larvae diets augmented with 31 different amino compounds, administered in different concentrations, and observed their effects on larval metabolomic composition, viability, rate of development and freeze tolerance...

...Bradley L. Baumgarner; Bruce R. Cooper SUMMARY This study was conducted to evaluate the use of a two-dimensional gas chromatography/time-of-flight mass spectrometry (GC×GC/TOF-MS) metabolomic platform to comprehensively analyze the effect of starvation on whole-animal metabolism in rainbow trout...