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Keywords: membrane potential
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Journal Articles
J Exp Biol (2021) 224 (10): jeb238147.
Published: 28 May 2021
... by 10% with the onset of anoxia in goldfish telencephalic brain slices. Employing whole-cell patch-clamp recording, we found that, similar to severe hypoxia, the ROS scavengers N -acetyl cysteine (NAC) and MitoTEMPO, added during normoxic periods, depolarized membrane potential (severe hypoxia −73.6...
Journal Articles
J Exp Biol (2018) 221 (4): jeb172213.
Published: 14 February 2018
... and an upregulation of oxidative phosphorylation efficiency. The aim of this study was to decipher mechanisms underlying the increased mitochondrial coupling efficiency reported in fasted birds. Mitochondrial oxidative phosphorylation activity, efficiency and membrane potential were measured in mitochondria isolated...
Journal Articles
J Exp Biol (2017) 220 (4): 695–704.
Published: 15 February 2017
..., we recorded the electrical activities of both excitatory (pyramidal) and inhibitory (stellate) neurons. With anoxia, membrane potential ( V m ) depolarized in both cell types from −72.2 mV to −57.7 mV and from −64.5 mV to −46.8 mV in pyramidal and stellate neurons, respectively. While pyramidal cells...
Journal Articles
J Exp Biol (2016) 219 (16): 2504–2513.
Published: 15 August 2016
Includes: Supplementary data
Journal Articles
J Exp Biol (2014) 217 (8): 1297–1306.
Published: 15 April 2014
... have been associated with a loss and recovery of ion homeostasis (particularly extracellular [K + ], [K + ] o ) and accordingly onset of chill coma has been hypothesized to result from depolarization of membrane potential caused by loss of ion homeostasis. Here, we examined whether onset of chill coma...
Includes: Supplementary data
Journal Articles
J Exp Biol (2010) 213 (23): 3980–3987.
Published: 01 December 2010
...Heidi K. Grønlien; Anna Kari Rønnevig; Bjarne Hagen; Olav Sand SUMMARY The swimming behaviour of ciliates is mainly determined by membrane potential and transmembrane fluxes. In a chemical gradient, swimming ciliates may approach or move away from the source. Based on experiments on Paramecium...
Journal Articles
J Exp Biol (2009) 212 (2): 270–276.
Published: 15 January 2009
.... * Author for correspondence (e-mail: nakaoka@bpe.es.osaka-u.ac.jp ) 13 11 2008 2009 spontaneous fluctuation membrane potential calcium Paramecium Paramecium multimicronucleatum cells were cultured in a hay infusion inoculated with Klebsiella pneumoniae . The culture...
Journal Articles
J Exp Biol (2005) 208 (20): 3957–3969.
Published: 15 October 2005
..., despite being derived from different parts of the contractile vacuole complex. No voltage-gated unit currents were observed in membrane patches from the vesicles. Vesicles derived from the radial arm showed a membrane potential of >10 mV, positive with reference to the cytosol, while those derived from...
Journal Articles
J Exp Biol (2005) 208 (13): 2569–2579.
Published: 01 July 2005
... for pH 8 and 9. Plasma membrane potential differences were measured for cells growing in MAM at pH 2 and pH 7 without trace elements or vitamins. The cell holding chamber consisted of two 24×60 mm coverslips spaced 3-3.5 mm apart by three standard sized microscope slides at each end, allowing...
Journal Articles
J Exp Biol (2004) 207 (12): 1977–1983.
Published: 15 May 2004
..., is involved in ammonia toxicity. * Author for correspondence (e-mail: BHRAND@cityu.edu.hk ) 26 1 2004 © The Company of Biologists Limited 2004 2004 oriental weatherloach Misgurnus anguillicaudatus ammonia glutamine alanine K + channel membrane potential NMDA receptor...
Journal Articles
J Exp Biol (2003) 206 (6): 949–957.
Published: 15 March 2003
...U. I. M. Wiehart; S. W. Nicolson; E. Van Kerkhove SUMMARY Malpighian tubules of the mealworm Tenebrio molitor were isolated for intracellular measurement of basolateral ( V bl ) and,indirectly, apical ( V ap ) membrane potentials. In control Ringer (50 mmol l -1 K + , 140 mmol l -1 Na + ), V bl...
Journal Articles
J Exp Biol (2002) 205 (20): 3261–3270.
Published: 15 October 2002
...: naitoh@pbrc.hawaii.edu ) 18 7 2002 © The Company of Biologists Limited 2002 2002 contractile vacuole complex membrane potential V-ATPase osmoregulation fluid segregation activity exocytotic cycle Paramecium multimicronucleatum The contractile vacuole complex (CVC...
Journal Articles
J Exp Biol (2001) 204 (15): 2691–2698.
Published: 01 August 2001
... ) was significantly lower in ammonia-exposed animals, leading to a profound reduction in factorial aerobic scope (AMR/MMR). Reduced U crit was also linked to a reduction in maximum tailbeat frequency. Calculation of membrane potentials ( E M ) in the white muscle of fish swum to U crit revealed a significant partial...
Journal Articles
J Exp Biol (1991) 161 (1): 239–255.
Published: 01 November 1991
... is highly useful for the analysis of ciliary electromotor coupling. The functions of intraciliary Ca 2+ in the regulation of the motor responses are discussed. 17 7 1991 © 1991 by Company of Biologists 1991 membrane potential voltage-clamp cirral movement Stylonychia mytilus /. exp...
Journal Articles
J Exp Biol (1989) 144 (1): 43–51.
Published: 01 July 1989
... the membrane potential. The depolarization was larger in the night cells than in the day cells. The resting potential measured in the dark was about 4 mV more positive in the night cells than in the day cells. Membrane conductance was larger in the day than at night, which can explain the changes...
Journal Articles
J Exp Biol (1988) 137 (1): 477–485.
Published: 01 July 1988
... depolarization of membrane potential, and a step-down caused recovery to the original level. The action spectrum of the depolarization corresponded to that for phototaxis, and showed two peaks at 420 and 560 nm. The amplitude of the depolarization became larger as light intensity was increased. Cells which...
Journal Articles
J Exp Biol (1988) 136 (1): 149–175.
Published: 01 May 1988
... ammonia levels were equal to those predicted by the plasma to red cell pH gradient. Red cells are unusual in that hydrogen ions are passively distributed according to membrane potential (E M ), whereas in other tissues this is not the case. In white muscle, heart and brain under all experimental...
Journal Articles
J Exp Biol (1988) 135 (1): 25–38.
Published: 01 March 1988
...DAVID F. MOFFETT; ALAN R. KOCH Basal membrane potential (V b ) and intracellular K + activity [(K + ) i ] were recorded, using microelectrodes, in isolated, superfused, short-circuited midgut of fifth instar larvae of Manduca sexta . The electrochemical gradient across the basal membrane...
Journal Articles
J Exp Biol (1988) 135 (1): 39–49.
Published: 01 March 1988
..., and not a concentrationdependence of the pump, that exercises the major role in determining the relationship between extracellular (K + ) and net K + transport by the isolated gut. 2 9 1987 © 1998 by Company of Biologists 1998 Manduca sexta midgut K + transport goblet cell membrane potential ion-specific...
Journal Articles
J Exp Biol (1987) 131 (1): 107–115.
Published: 01 September 1987
...YASUO NAKAOKA; KOHSAKU KINUGAWA; TOHRU KUROTANI Photostimulation of deciliated specimens of chlorella-free P. bursaria elicited a transient depolarization of the membrane potential. The amplitude of this receptor potential became larger as light intensity was increased and the relationship showed...