...Jonathan C. Radford; Selim Terhzaz; Pablo Cabrero; Shireen-A. Davies; Julian A. T. Dow SUMMARY Identification of the Anopheles gambiae leucokinin gene from the completed A. gambiae genome revealed that this insect species contains three leucokinin peptides, named Anopheles leucokinin I-III...

...Ming-Jiun Yu; Klaus W. Beyenbach SUMMARY The diuretic peptide leucokinin is known to increase fluid secretion in Malpighian tubules of the yellow fever mosquito Aedes aegypti by increasing a transepithelial Cl - conductance. The present study sought to examine whether stellate cells provided...

.... The intracellular messenger of MNP is cAMP, which increases the Na + conductance and activates the Na + /K + /2Cl - -cotransporter in the basolateral membrane of principal cells. Leucokinin non-selectively increases transepithelial NaCl and KCl secretion, which may deal with hemolymph volume expansions or reduce...

... melanogaster renal (Malpighian) tubules. Intact tubules from norpA mutants severely reduced diuresis stimulated by the principal cell- and stellate cell-specific neuropeptides, CAP 2b and Drosophila leucokinin (Drosokinin), respectively, suggesting a role for PLCβ in both these cell types. Measurement of IP 3...

... bioassay, we show that three tachykinin-related peptides (TRPs),leucokinin I, serotonin (5-HT), octopamine, the cardioacceleratory peptides 1a, 1b and 2c, cGMP and cAMP each cause an increase in the rate of fluid secretion in pharate adult tubules. Whereas the possible hormonal sources of biogenic amines...

..., are detectable in the tubule by reverse transcription–polymerase chain reaction. To identify the cell type responsible for the oscillations, tubules were treated with diuretic hormones known to alter calcium levels in each of the two cell types. Leucokinin-IV, which increases calcium levels in the stellate cells...

...Selim Terhzaz; F. Claire O’Connell; Valerie P. Pollock; Laura Kean; Shireen A. Davies; Jan A. Veenstra; Julian A. T. Dow ABSTRACT The leucokinin (LK) family of neuropeptides has been found widely amongst invertebrates. A member of this family was purified from adults of the fruit fly Drosophila...

... with leucokinin, does not affect the potential profile across the principal cells, showing that chloride must pass through another route. The cell-permeant Ca 2+ chelator BAPTA-AM was shown to suppress the action of leucokinins (insect peptides that induce rapid fluid secretion), but not those of cyclic AMP...