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Keywords: intracellular pH
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Journal Articles
J Exp Biol (2018) 221 (10): jeb172551.
Published: 25 May 2018
... about bimodal breathers, which utilize both water and air. Here, we characterized the influence of temperature on arterial acid–base balance and intracellular pH (pH i ) in the bimodal-breathing swamp eel, Monopterus albus . This teleost uses the buccopharyngeal cavity for gas exchange and has very...
Journal Articles
J Exp Biol (2012) 215 (8): 1337–1345.
Published: 15 April 2012
...). Whether and to what extent V-ATPase contributes to intracellular pH (pH i ) regulation in unstimulated gland cells is unknown. We used the fluorescent dye BCECF to study intracellular pH i regulation microfluorometrically and show that: (1) under resting conditions, the application of Na + -free...
Journal Articles
J Exp Biol (2009) 212 (16): 2571–2578.
Published: 15 August 2009
... with submicromolar serotonin. In the present study we investigated the mechanisms of strong luminal alkalinization using the intracellular fluorescent indicator BCECF-AM. Following stimulation with serotonin, we observed that intracellular pH (pH i ) of the anterior midgut increased from a mean of 6.89 to a mean...
Journal Articles
J Exp Biol (2009) 212 (9): 1270–1276.
Published: 01 May 2009
... cardiac performance was restored in six out of seven hearts upon return to control conditions. Myocardial intracellular pH (pH i ) was protected in situ , as has been found in vivo , and this protection extended to the highest level of CO 2 (7.5%)investigated. Thus, maintained heart function during...
Journal Articles
J Exp Biol (2008) 211 (5): 805–815.
Published: 01 March 2008
... microfluometrically whether V-ATPase and carbonic anhydrase (CA) are involved in intracellular pH (pH i ) regulation,and we have localized CA activity by histochemistry. We show: (1) mean pH i in salivary gland cells is 7.5±0.3 pH units( N =96), higher than that expected from passive H + distribution; (2) low 5-HT...
Journal Articles
J Exp Biol (2008) 211 (4): 568–576.
Published: 15 February 2008
... peripheral cells and in the basolateral plasma membrane domain of duct cells. In addition, duct cells have been shown to have a V-H + -ATPase in their apical membrane( Just and Walz, 1994b ). Other studies have recorded intracellular pH i ,[Na + ] i and [Ca 2+ ] i in duct cells of isolated lobes ( Lang...
Journal Articles
J Exp Biol (2007) 210 (21): 3749–3756.
Published: 01 November 2007
... by a significant increase in inorganic phosphate levels from 0.3±0.3 to 9.5±3.4 mol g –1 , and a drop in intracellular pH from 7.48±0.03 to 6.81±0.05 in muscle. This coincides with the point when the fish change gait from subcarangiform swimming to kick-and-glide bursts. As the number of kicks increased, so too...
Journal Articles
J Exp Biol (2007) 210 (19): 3473–3483.
Published: 01 October 2007
... in intracellular pH (pHi) that was dependent on Ca 2+ influx from the extracellular medium as well as Ca 2+ release from intracellular pools. Surprisingly, this increase in pHi and intracellular Ca 2+ concentration,[Ca 2+ ] i , was not accompanied by any change in proton secretion. By contrast, removal...
Journal Articles
J Exp Biol (2007) 210 (8): 1463–1471.
Published: 15 April 2007
...Carsten Hille; Bernd Walz SUMMARY Cells of the dopaminergically innervated salivary ducts in the cockroach Periplaneta americana have a vacuolar-type H + -ATPase(V-ATPase) of unknown function in their apical membrane. We have studied whether dopamine affects intracellular pH (pH i ) in duct cells...
Journal Articles
J Exp Biol (2006) 209 (16): 3101–3113.
Published: 15 August 2006
...Khaled H. Ahmed; Bernd Pelster; Gerhard Krumschnabel SUMMARY In trout hepatocytes, hypertonicity and cytosolic acidification are known to stimulate Na + /H + exchanger (NHE) activity, which contributes to recovery of cell volume and intracellular pH (pHi),respectively. The present study...
Journal Articles
J Exp Biol (2006) 209 (10): 1964–1975.
Published: 15 May 2006
... increase in lumen pH to 6.32±0.04 ( N =5). Intracellular pH was 6.97±0.01 and 6.82±0.04 in unstimulated and serotonin-stimulated tubules, respectively. Lumen pH was altered whereas intracellular pH was tightly regulated during serotonin and bumetanide treatment. Furthermore, DIDS or amiloride treatment did...
Journal Articles
J Exp Biol (2006) 209 (8): 1560–1572.
Published: 15 April 2006
... -311. Browning, J. A. and Wilkins, R. J. ( 2004 ). Mechanisms contributing to intracellular pH homeostasis in an immortalised human chondrocyte cell line. Comp. Biochem. Physiol. 137A , 409 -418. Burkman, L. J., Overstreet, J. W. and Katz, D. F. ( 1984 ). A possible role for potassium...
Journal Articles
J Exp Biol (2005) 208 (23): 4411–4418.
Published: 01 December 2005
...Ayako Nakajima; Masaya Morita; Akihiro Takemura; Shinji Kamimura; Makoto Okuno SUMMARY Increased intracellular pH ([pH] i ) activates dynein in sea urchin and mammalian sperm and induces activation of flagellar motility. It is thought that cAMP-dependent protein phosphorylation is associated...
Journal Articles
J Exp Biol (2005) 208 (21): 4137–4149.
Published: 01 November 2005
..., and a recovery only limited by the physical stability of the utilized optical system. Intracellular pH (pH i ) in Danio rerio embryos between 1k-cells stage and the end of epiboly was found to be well regulated to a mean value of 7.55±0.13 (± s . d .), a range distinctly more alkaline than typical values...
Journal Articles
J Exp Biol (2005) 208 (14): 2799–2808.
Published: 15 July 2005
... et al., 1982 ; Kwast et al., 1995 ). Intracellular pH was estimated from the chemical shift (resonance frequency)of P i using a titration curve generated in our facility( Fig. 2C ). This standard curve was produced using a solution of 10 mmol l –1 P i ,110 mmol l –1 KCl and 20 mmol l –1 NaCl...
Journal Articles
J Exp Biol (2003) 206 (20): 3681–3692.
Published: 15 October 2003
... downregulation, characterized by a steep drop in intracellular pH (pH i ) and ATP levels. Using dot blots of total mitochondrial RNA, we show that during in organello incubations both O 2 deprivation and acidic pH (pH 6.4) elicit increases in half-lives of selected mitochondrial transcripts on the order of five...
Journal Articles
J Exp Biol (2002) 205 (8): 1153–1160.
Published: 15 April 2002
... ratios in non-perfused preparations of body wall musculature at various levels of extra- and intracellular pH, P CO 2 and [HCO 3 - ]. A decrease in extracellular pH from control level (7.9) to 6.7 caused a reduction in aerobic metabolic rate of both normocapnic and hypercapnic tissues by 40-45 %. O/N...
Journal Articles
J Exp Biol (2001) 204 (22): 3943–3951.
Published: 15 November 2001
...Gerhard Krumschnabel; Claudia Manzl; Pablo J. Schwarzbaum SUMMARY Mechanisms of intracellular pH (pHi) regulation were investigated in anoxia-tolerant hepatocytes from goldfish Carassius auratus , and compared to the situation in the anoxia-intolerant hepatocytes from trout Oncorhynchus mykiss...
Journal Articles
J Exp Biol (1992) 169 (1): 235–249.
Published: 01 August 1992
... pathway of ammonia excretion through fish muscle membranes. Note: To whom reprint requests should be sent. 27 4 1992 © 1992 by Company of Biologists 1992 ammonia carbon dioxide intracellular pH acid-base white muscle rainbow trout Oncorhynchus mykiss exercise /. exp. Biol...
Journal Articles
J Exp Biol (1991) 159 (1): 387–405.
Published: 01 September 1991
...AREN VAN WAARDE; INGRID DE GRAAFF; GUIDO VAN DEN THILLART; CEES ERKELENS Goldfish acclimated to 20 and 5°C were subjected to anoxia (3 h at 20°C and 18 h at 5°C). The intracellular pH (pHi) and the levels of high-energy phosphate compounds in the epaxial muscle were continuously monitored...