... in a competitive manner with ammonia. In situ hybridization experiments showed that both transporters were expressed as early as the 4-cell stage in zebrafish embryos and could be identified in most tissues 4 days post-fertilization. Larvae experiencing morpholino-mediated knockdown of DrHiat1b exhibited...

... use, distribution and reproduction in any medium provided that the original work is properly attributed. Amphibians Olfactory organ RT-PCR In situ hybridization The organization of olfactory organs varies considerably across vertebrate species. Fishes generally possess a single...

... in the same stemma. * Author for correspondence ( elke.buschbeck@uc.edu ) 22 8 2009 © 2009. 2009 stemmata eye in situ hybridization visual pigments visual system insects Adult insects are generally characterized by compound eyes, and the vast majority of the research...

... of SWS1 opsin expression in the retina of adult rainbow trout, perhaps via a reverse process to that occurring in the juvenile, has not been investigated. Here, we used in situ hybridization with species-specific riboprobes and microspectrophotometry on rainbow trout retina to show that: (1) single cones...

..., including the novel insect amino acid transporter subfamily, or into a group of weakly related sequences not affiliated with a recognized subfamily. Reverse transcription-polymerase chain reaction analysis and in situ hybridization showed that seven of these genes are expressed in the CNS. In situ...

... in function in some species. Rescan and Gauvry ( 1996 ) isolated a second form of MyoD from the trout, and demonstrated different expression patterns using in situ hybridization. MyoD1 was expressed in the adaxial cells of the unsegmented mesodermal plate and in the developing somites. MyoD2...

...Thomas E. Hall; Nicholas J. Cole; Ian A. Johnston SUMMARY Seven cDNA clones coding for different muscle-specific proteins (MSPs) were isolated from the fast muscle tissue of Atlantic cod Gadus morhua L. In situ hybridization using cRNA probes was used to characterize the temporal and spatial...

... 2002 © The Company of Biologists Limited 2003 2003 Vestimentifera carbonic anhydrase V-H + ATPase Na + K + -ATPase immunolocalization in situ hybridization Carbonic anhydrases (CAs) are important enzymes (E.C.4.2.1.1) commonly produced by a variety of tissues, where...

... to cytochalasin B and phloretin and kinetic parameters showed that the rainbow trout glucose transporter was similar to a carp transporter and to mammalian GLUT1. Embryonic expression of OnmyGLUT1 was studied using whole-mount in situ hybridization. Ubiquitous distribution of transcripts was observed until...

.... Digoxigenin-labelled RNA probes were used for in situ hybridization of V-ATPase mRNA of both peripheral and integrated subunits; monoclonal antibodies to subunits of the peripheral sector of the purified plasma membrane V-ATPase were used for immunocytochemistry. Extensive mRNA labelling was found in both...

... of structure–function relationships in heterologous expression systems and a biophysical analysis of transporter function. In situ hybridization and immunocytochemical studies indicate a primary expression of NET and SERT genes in brain by noradrenergic and serotonergic neurons, respectively. Both NET and SERT...

... provide a powerful tool for investigating the control of transmitter and receptor physiology at any site in the body which responds to its local chemical environment. © 1988 by Company of Biologists 1988 opioids stress in situ hybridization hypothalamus. The hypothalamic...