... for sunfish biology. Comparative analyses of the transcriptomes revealed an unexpected, bluegill-specific expansion in the HSP70 and HSP90 molecular chaperone gene families. These expansions were not unique to the bluegill as expansions in HSP70s and HSP90s were identified in the genomes of other teleost fish...

... to ecologically representative diel hypoxia cycles improved hypoxia tolerance. Diel hypoxia-induced protective responses included increased inducible HSP70 concentration and mean corpuscular hemoglobin concentration, as well as reduced plasma cortisol. Acclimation to diel hypoxia allowed metabolic rates...

... homeostasis Hydrostatic pressure HSP70 Physiology Stress, and the way organisms respond to stressors, is undoubtedly one of the most important concepts in biology, influencing an organism's physiology at all levels of organisation from molecular to whole organism, as well as its ecology ( Bjilsma...

... shock protein ( hsp70 ) mRNA expression. Basal plasma and erythrocyte nitrite levels were higher in hypoxia than normoxia, suggesting increased NOS activity. Nitrite exposure strongly elevated nitrite concentrations in plasma, erythrocytes, heart tissue and white muscle, which was associated...

... protein 70 (Hsp70) induction, both essential mechanisms of the heat stress response. We exposed snails to elevated temperature (25, 38, 40, 43 and 45°C) in the laboratory and measured the activity of the antioxidant enzymes catalase (CAT) and glutathione peroxidase (GPx), determined the Hsp70 level...

... functionally different skeletal muscles, the gastrocnemius (jumping muscle) and the iliofibularis (non-jumping muscle), by aestivating frogs at 24 and 30°C for 6 months. We assayed small molecule antioxidant capacity, mitochondrial and cytosolic superoxide dismutase activities and Hsp70 concentrations to show...

... in so-called ramping assays. We exposed flies to ramping at rates of 0.06 and 0.1°C min -1 , respectively. Flies were sampled from the two treatments at 28, 30, 32, 34, 36 and 38°C and tested for heat tolerance and expression levels of the heat shock genes hsp23 and hsp70 , as well as Hsp70 protein...

... consequently can serve as a stress indicator (similar to heat shock proteins,HSPs). We tested these hypotheses through two experiments with the rock crab, Cancer irroratus . First, crabs were exposed to a progressive temperature increase (6°C h –1 ) from 12 to 30°C. AMPK activity, total AMPK protein and HSP70...

... from the extracellular space remains unclear. Using two well-characterized cell types from the sea urchin,we show that extracellular mixtures of the constitutive and inducible forms of the 70 kDa heat shock proteins (Hsc70 and Hsp70, respectively) have dramatic effects on initiation of cell division...

... shock protein. Here we examine genetic, behavioral and environmental factors affecting a performance character, running speed, for willow beetles, and assess effects of consecutive cold and heat exposure on running speed and expression of Hsp70 in the laboratory. In nature, running speed depends on air...

..., and a marker for the cellular stress response, inducible heat shock protein 70 (Hsp70), was assayed by immunoblot analysis. Transient heat stress (a shift from the normal 13.5°C to 23.5°C for 1 h) decreased 2,4-D transport by ∼66%;however, the same stress minus TMAO (isosmotic replacement with urea) had...

... that the relationships between basal and inducible thermotolerance are contingent upon the methods used to gauge thermotolerance,as well as the sex of the flies. Finally, we compared temporal profiles of the combined expression of two major heat-shock proteins, HSC70 and HSP70, during heat stress among the females...

... in tissue-specific pro- or anti-apoptotic events revealed that identical stressful stimuli possibly lead to apoptotic death via the caspase-3 activation in the mantle tissue and to anti-apoptotic events possibly via the induction of Hsp70 overexpression in the gill tissue. * Author for correspondence...

... stages of the hsp gene expression pathway, including transcription factor activity, Hsp70 mRNA production and protein synthesis patterns, in hepatocytes from T. bernacchii . Hsp70 mRNA was detected,as was heat shock factor 1 (HSF1) with DNA-binding activity. However, exposure to elevated temperature...

...Rona G. Giffard; Lijun Xu; Heng Zhao; Whitney Carrico; Yibing Ouyang; Yanli Qiao; Robert Sapolsky; Gary Steinberg; Bingren Hu; Midori A. Yenari SUMMARY Chaperones, especially the stress inducible Hsp70, have been studied for their potential to protect the brain from ischemic injury. While...

... described partial sequences of three heat-shock protein ( hsp70 family) genes and examined gene expression on the way from an active to a cryptobiotic and back to an active stage again. Results showed different patterns of gene expression in the hsp70 isoforms. All three isoforms seem to be true heat-shock...

...-shock response. The low-latitude species D. virilis exceeds the high-latitude species D. lummei in these measures of thermotolerance, the temperature threshold for heat-shock factor (HSF)activation and the ability to express hsp70 mRNA and diverse heat-shock proteins (e.g. Hsp70, Hsp83 and small Hsps...

... levels of heat-shock proteins of the 40, 70 and 90 kDa families (hsp 40, hsp70 and hsp90, respectively) would be predicted to elevate T on . Conversely, elevated levels of HSF1 would be predicted to decrease T on . Following laboratory acclimation to 13, 18 and 23°C, we used solid-phase immunochemistry...

...S. C. Lakhotia; K. V. Prasanth SUMMARY The haploid genome of Drosophila melanogaster normally carries at least five nearly identical copies of heat-shock-inducible hsp70 genes, two copies at the 87A7 and three copies at the 87C1 chromosome sites. We used in situ hybridization of the cDNA, which...

...Yueh-Tsu King; Chih-Sheng Lin; Jyh-Hung Lin; Wen-Chuan Lee SUMMARY Molecular mechanisms of whole-body thermotolerance (WBT) in mammals have not been investigated thoroughly. The purpose of this study was to assess the induction of the 70 kDa heat shock protein (HSP70) and antioxidant enzyme...