... cation antiporter and a low affinity, high capacity organic anion exchanger. Together these two systems have the potential to regulate cytoplasmic metals over a wide concentration range. The crustacean hepatopancreas is a multifunctional organ that is composed of hundreds of blind-ending tubules lined...

.... The majority of the genes were downregulated, presumably by an energetic shift of the hepatopancreas prior to ecdysis. The effect of 20-hydroxyecdysone on representative genes from this group was confirmed in vitro using a hepatopancreas tissue culture. This approach for ecdysteroid-responsive gene...

... in hepatopancreas, expression in gill was 10-fold greater and expression was highest in antennal gland (15-fold greater than in hepatopancreas). Compared with baseline expression levels in intermolt stage,expression of ECaC in antennal gland increased 7.4- and 23.8-fold,respectively, in pre- and postmolt stages...

... membrane Na + /K + -ATPase is a potentially key target for regulation during estivation. Na + /K + -ATPase was investigated in foot muscle and hepatopancreas of the land snail Otala lactea , comparing active and estivating states. In both tissues enzyme properties changed significantly during estivation...

... was recorded for Buthidae following severe desiccation. When desiccated, scorpions lose water primarily from the hepatopancreas, while haemolymph volume is more tightly regulated. However,the haemolymph volume of Scorpionidae decreases as a result of depletion of hepatopancreas water stores following severe...

...George A. Gerencser; Frank Robbins; Jianliang Zhang; Gregory A. Ahearn SUMMARY The transport of [ 14 C]oxalate (Ox 2–) by epithelial brush-border membrane vesicles (BBMV) of lobster (Homarus americanus) hepatopancreas, formed by a magnesium precipitation technique, was stimulated by an outward Cl...

...Pamela Chavez-Crooker; Nestor Garrido; Gregory A. Ahearn SUMMARY Mechanisms of copper transport into purified mitochondrial suspensions prepared from the hepatopancreas of the Atlantic lobster Homarus americanus were investigated. Mitochondria were purified by combining methods of differential...

...P. Chavez-Crooker; N. Garrido; G. A. Ahearn ABSTRACT The hepatopancreas of the American lobster (Homarus americanus) possesses four types of epithelial cells arranged along blind-ended tubules. At the distal tips of these tubules, stem cells termed E-cells differentiate into three other cell types...

...T. Bishop; M. D. Brand ABSTRACT Cells isolated from the hepatopancreas of the land snail Helix aspersa strongly depress respiration both immediately in response to lowered (oxygen conformation) and, in the longer term, during aestivation. These phenomena were analysed by dividing cellular...

... © 2000 by Company of Biologists 2000 sulphate/oxalate exchange hepatopancreas binding constant Atlantic lobster Homarus americanus Gastrointestinal and renal transport of the divalent anion sulfate across epithelial apical membranes has been investigated in various vertebrate...

...Mahealani K. Monteilh-Zoller; Vincenzo Zonno; Carlo Storelli; Gregory A. Ahearn ABSTRACT Epithelial brush-border membrane vesicles (BBMVs) from the hepatopancreas of the lobster Homarus americanus were prepared using a magnesium precipitation technique and employed in transport experiments designed...

...Fabrice Durand; Fabienne Chausson; Michèle Regnault ABSTRACT Carcinus maenas and Necora puber were exposed to air for 72 h and 18 h, respectively, at 18 °C. Changes in the free amino acid (FAA) content of their muscle, hepatopancreas and haemolymph were recorded during air-exposure and subsequent...

... of crustacean antennal glands . Am. J. Physiol. 264 , R1206 – R1213 . Ahearn , G. A. , Grover , M. L. , Tsuji , R. T. and Clay , L. P. (1987). Proton-stimulated Cl–HCO3 antiport by basolateral membrane vesicles of lobster hepatopancreas . Am. J. Physiol. 252 , R859 – R870...

... was subtracted from total uptake at selected exposure intervals to provide estimates of transport activity alone. Fig. 1. The effect of a transmembrane osmotic gradient on equilibrium uptake of 45 Ca 2+ by brush-border membrane vesicles (BBMVs) from lobster hepatopancreas. Vesicles were loaded with 200...

...M. Thamotharan; G. A. Ahearn ABSTRACT Epithelial brush-border membrane vesicles (BBMVs) of lobster (Homarus americanus) hepatopancreas were formed by a Mg 2+ precipitation technique. In these BBMVs, [ 14 C]glycylsarcosine ([ 14 C]Gly-Sar) uptake was stimulated by a transmembrane proton gradient...

... 2Na/1H antiport in crustacean hepatopancreas . Am. J. Physiol . 257 , R484 – R493 . Ahearn , G. A. and Franco , P. (1990). Na and Ca share the electrogenic 2Na/1H antiporter in crustacean antennal glands . Am. J. Physiol . 259 , F758 – F767 . Ahearn , G...

... of the invertebrate electrogenic cation exchanger, may be diverse. References Ahearn , G. A. and Clay , L. P. (1989). Kinetic analysis of electrogenic 2Na + /1H + antiport in crustacean hepatopancreas . Am. J. Physiol . 257 , R484 – R493 . Ahearn , G. A. and Franco , P. (1990...

...Teva Siu; Gregory A. Ahearn ABSTRACT The mechanism of [ 3 H]myo-inositol transport by the lobster hepatopancreas was examined using purified brush-border membrane vesicles. Transport was stimulated by a 100 mmol 1 −1 inward Na + gradient, but other cation gradients were ineffective, suggesting a Na...

... from diffusion, was Na + -and Cl − -independent, and was cis-inhibited by more polar amino acids such as lysine, hydroxyproline, MeAIB, alanine and glutamate. These results suggest that brush-border leucine transport in lobster hepatopancreas at acidic pH may occur by a combination of a modified L...

... gastrointestinal physiology Homarus americanus hepatopancreas Amino acid transport across epithelial membranes occurs by a variety of carrier processes that illustrate various degrees of substrate specificity and are either sodiumdependent or sodium-independent (Stevens, Ross & Wright, 1982). While...