... stages of the hsp gene expression pathway, including transcription factor activity, Hsp70 mRNA production and protein synthesis patterns, in hepatocytes from T. bernacchii . Hsp70 mRNA was detected,as was heat shock factor 1 (HSF1) with DNA-binding activity. However, exposure to elevated temperature...

...Paul L. Else; M. D. Brand; N. Turner; A. J. Hulbert SUMMARY Hepatocytes were isolated from eight species of birds ranging from 13 g zebra finches to 35 kg emus. This represents a 2800-fold range of body mass( M b ). Liver mass (g) was allometrically related to species body mass by the equation...

... a general stress factor in aquatic environments, with largely unknown effects on species survival and well being, especially in cold and deep waters. For an analysis of CO 2 effects at the cellular level, isolated hepatocytes were prepared from two representatives of the Antarctic fish fauna, Pachycara...

...-mail: [email protected] ) 18 3 2003 © The Company of Biologists Limited 2003 2003 V-ATPase subunit B isoform hepatocyte expression zebrafish Danio rerio. In eukaryotic organisms vacuolar-type ATPase (V-ATPase) is a proton pump located in cytoplasmatic membranes...

...Gerhard Krumschnabel; Ronald Gstir; Claudia Manzl; Caroline Prem; Diego Pafundo; Pablo J. Schwarzbaum SUMMARY Trout hepatocytes exposed to hypo- or hyperosmotic conditions respond by swelling and shrinking, respectively, followed by regulatory volume changes that almost, although not completely...

...M. V. Espelt; P. N. Mut; G. Amodeo; G. Krumschnabel; P. J. Schwarzbaum SUMMARY The relationship between cell volume and K + transmembrane fluxes of goldfish ( Carassius auratus ) hepatocytes exposed to anisotonic conditions or energetic limitation was studied and compared with the response...

... to the mechanism of multidrug resistance exhibited in chemotherapy-resistant human tumor cells. In the present study, a variety of techniques were used to characterize this toxin defense system in killifish ( Fundulus heteroclitus ) hepatocytes. The cellular localization and activity of the putative drug efflux...

... size class of heat-shock protein. Parallel in vivo labelling experiments with isolated hepatocytes similarly showed significant amounts of protein synthesis, but no indication of enhanced expression of any class of hsp. The heavy metal cadmium, which is known to induce synthesis of hsps, also failed...

...M. Furimsky; T. W. Moon; S. F. Perry ABSTRACT The mechanisms of intracellular pH (pHi) regulation were examined in hepatocytes of the rainbow trout Oncorhynchus mykiss . pHi was monitored using the pH-sensitive fluorescent dye BCECF, and the effects of various media and pharmacological agents were...

...Susan J. Mckinley; Jeffrey R. Hazel ABSTRACT The potential role of compensatory adjustments to membrane components in determining the function of the β-adrenergic receptor/adenylyl cyclase (β-AR/AC) signal-transduction system was studied in isolated hepatocytes of 5 °C- and 20 °C-acclimated rainbow...

... ). In mammalian hepatocytes, food deprivation induces the appearance of a high-affinity component for short-chain amino acid transport, with the properties of system A, while there are no changes in the activities of a low-affinity system (the ASC system), system L ( Fehlmann et al . 1979 ) or glutamine uptake...